| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | 8 hydroxyguanine, 8-OH-dG, 8-OHdG, 80G, 8OHG, DNA Damage |
| Assay Type | |
| Detection Method | |
| Detection Range | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Urine, Cell Lysates, Plasma, Sample matrices |
| Sensitivity | |
| Shipping | |
| Storage | |
| Target |
8-hydroxy-2'-deoxyguanosine (8-OHdG) is a widely recognized biomarker of oxidative DNA damage, formed when reactive oxygen and nitrogen species modify guanine bases. This hydroxylation process occurs during normal metabolism and is amplified by environmental stressors that increase oxidative load. Elevated levels of 8-OHdG are strongly associated with aging and a range of chronic conditions, including cancer, diabetes, hypertension, and neurodegenerative diseases. In neuroscience, 8-OHdG serves as a critical indicator of oxidative stress in brain tissue, where neurons are particularly vulnerable due to high metabolic activity and lipid content. Increased 8-OHdG levels have been linked to the progression of Alzheimer’s, Parkinson’s, and other neurodegenerative disorders, making it a valuable tool for monitoring disease onset and therapeutic response. 8-OHdG can exist as a free nucleoside or be incorporated into DNA. In biological samples such as plasma, cell lysates, and tissues, its detection is complex; however, urine provides a more reliable matrix for measuring free 8-OHdG due to efficient renal filtration. Typical urinary concentrations range from 2.7–13 ng/mg creatinine, while plasma levels are significantly lower, often between 4–21 pg/mL as measured by LC-MS. By tracking 8-OHdG levels, researchers gain insight into cellular oxidative damage, enabling early detection and evaluation of antioxidant-based interventions in neurodegenerative disease research.
- Platform: Microplate
- Incubation Time: 1 hour
- Prepare standard and samples in the Sample and Standard Diluent.
- Add 50 µL of prepared standards and samples in triplicate to appropriate wells.
- Add 50 µL of the diluted antibody preparation to the appropriate wells.
- Cover plate with Plate Cover and incubate at room temperature (20-25°C) for 1 hour.
- Wash plate 4 times with 1X Wash Buffer.
- Add 100 µL of TMB Substrate to each well.
- Cover plate and develop the plate in the dark at room temperature for 30 minutes.
- Add 100 µL of Stop Solution to each well.
- Measure absorbance on a plate reader at 450 nm.
- Plot the standard curve and calculate sample concentrations.
| Component No. | Item | Quantity / Size |
|---|---|---|
| SKC-120A | 8-hydroxy-2-deoxy Guanosine : BSA Coated Plate | 1 Plate |
| SKC-120C | 8-hydroxy-2-deoxy Guanosine Standard | 1 vial/ 100uL |
| SKC-120F | 8-hydroxy-2-deoxy Guanosine HRP Conjugated Monoclonal Antibody | 1 vial/75uL |
| SKC-0001 | Sample and Standard Diluent | 1 vial/50mL |
| SKC-0002 | 8-hydroxy-2-deoxy Guanosine Antibody Diluent | 1 vial/13mL |
| SKC-0003 | Wash Buffer Concentrate | 1 vial/50mL |
| SKC-0004 | TMB Substrate | 1 vial/13mL |
| SKC-0005 | Stop Solution | 1 vial/13mL |
| SKC-0009 | Plate Cover | 2 covers |
DNA Damage (8-OHdG) ELISA Kit (StressMarq Biosciences, Canada, Cat # SKT-120-96S)
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