| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human DNAJA4 was used as the immunogen for the DNAJA4 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DNAJA4 Antibody / DnaJ homolog subfamily A member 4 is a anti-DNAJA4 Rabbit antibody Recombinant Rabbit Monoclonal clone 30D35 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DNAJA4
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30D35, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IP
Biological background
DnaJ homolog subfamily A member 4 contains a J domain that interacts with Hsp70 family chaperones to stimulate ATP hydrolysis and substrate binding. Research using DNAJA4 antibody has shown that it guides Hsp70 to specific client proteins, thereby influencing folding efficiency and preventing aggregation. This co-chaperone function is critical for maintaining proteostasis under normal and stress conditions.
Studies with DNAJA4 antibody have revealed important roles in cardiac and muscle physiology. DNAJA4 is enriched in cardiomyocytes, where it supports folding of contractile proteins and protects cells from stress-induced apoptosis. Loss of DNAJA4 function impairs sarcomeric organization and reduces cardiac performance. These findings highlight the relevance of DNAJA4 in cardiovascular health.
Dysregulation of DnaJ homolog subfamily A member 4 contributes to disease. Research using DNAJA4 antibody has shown that altered expression affects cell survival under stress, contributing to pathologies such as cardiomyopathy and neurodegeneration. Because DNAJA4 modulates stress response pathways, it is a candidate biomarker and therapeutic target in diseases linked to proteostasis failure.
DNAJA4 antibody is widely applied in western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects multiple isoforms and stress-induced expression, immunohistochemistry reveals tissue distribution in heart and muscle, and immunofluorescence demonstrates cytoplasmic localization associated with chaperone networks. These approaches make DNAJA4 antibody essential for research on molecular chaperones.
By providing validated DNAJA4 antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
