| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Concentration | |
| Conjugate | |
| Host | |
| Immunogen | Recombinant protein of human DNAJC15 |
| Isotype | |
| Product Type | |
| Shipping | |
| Storage | |
| UniProt # |
Product Overview
The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The presence of a J domain defines a protein as a member of the DnaJ family. DnaJ heat shock induced proteins are from the bacterium Escherichia coli and are under the control of the htpR regulatory protein. The DnaJ proteins play a critical role in the HSP 70 chaperone machine by interacting with HSP 70 to stimulate ATP hydrolysis. DnaJ proteins are important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. MCJ (methylation-controlled J protein), also known as HSD18, DNAJD1 or DNAJC15, is a 150 amino acid ubiquitously expressed single-pass membrane protein containing one J domain. Localizing to the golgi apparatus and only present in vertebrates, MCJ may be associated with increased chemotherapeutic resistance in ovarian cancer by inducing expression of the Mdr drug transporter and preventing intracellular drug accumulation.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Immunohistochemistry | IHC 1:50-1:200 |
Antibody Specifications
| Target | DNAJC15 |
|---|---|
| Host Species | Rabbit |
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugate | Unconjugated |
| Purification | Affinity Purification |
| Concentration | 0.3 mg/mL |
Immunogen
Recombinant protein of human DNAJC15 UniProt: Q9Y5T4.
Species Reactivity & Cross-Reactivity
Validated for: Human, Mouse. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles.
Storage Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4
Related Products
Browse additional antibodies targeting DNAJC15 and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
For IHC-P, heat-induced epitope retrieval (HIER) is typically required. Recommended starting conditions: citrate buffer pH 6.0 (microwave or pressure cooker, ~20 min) for rodent samples; EDTA buffer pH 9.0 for human clinical samples. After retrieval, allow sections to cool gradually before proceeding to blocking. Include a DNAJC15-positive tissue control (confirmed by literature or database expression data) and a negative isotype control in every run. Recommended dilution: IHC 1:50-1:200. Refer to the product datasheet for any target-specific retrieval guidance.
This antibody has been experimentally validated in: Human, Mouse. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "Recombinant protein of human DNAJC15" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
Store at -20℃ Valid for 12 months. Avoid freeze / thaw cycles. Storage Buffer: PBS with 0.05% sodium azide and 50% glycerol, PH7.4. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
Polyclonal antibodies are produced from immunized animals and may exhibit some degree of lot-to-lot variation in titer, background, and sensitivity. Each production lot is tested for performance against established acceptance criteria. When transitioning to a new lot, run a side-by-side pilot comparison against your previous lot using a validated positive control sample before updating your standard protocol. If significant performance differences are observed, contact our technical support team — titer adjustments may resolve the issue in most cases.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
