| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DNMT3A recombinant protein (Position: M1-Y284) was used as the immunogen for the DNMT3A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DNMT3A Antibody / DNA (cytosine-5)-methyltransferase 3A is a anti-DNMT3A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: DNMT3A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS, ELISA
Biological background
Functionally, DNMT3A antibody identifies an 912-amino-acid methyltransferase that functions together with DNMT3B and DNMT3L to establish DNA methylation marks during embryogenesis. DNMT3A binds chromatin through its PWWP domain and ADD (ATRX-DNMT3-DNMT3L) domain, recognizing histone modifications that guide locus-specific methylation. By controlling methylation of promoters, enhancers, and repetitive elements, DNMT3A ensures stable gene silencing and genome integrity.
The DNMT3A gene is located on chromosome 2p23.3 and is highly expressed in stem cells, germ cells, and hematopoietic progenitors. Expression decreases during differentiation, consistent with its role in developmental programming. DNMT3A activity is essential for imprinting, X-chromosome inactivation, and transposon silencing.
Pathologically, mutations in DNMT3A are among the most common in acute myeloid leukemia (AML), myelodysplastic syndromes, and clonal hematopoiesis. Loss-of-function mutations impair methylation fidelity, causing epigenetic instability and aberrant gene expression. Germline mutations lead to Tatton-Brown-Rahman syndrome, characterized by overgrowth and intellectual disability. Research using DNMT3A antibody supports studies in epigenetics, cancer biology, and developmental regulation.
DNMT3A antibody is validated for western blotting, immunofluorescence, and chromatin immunoprecipitation to detect DNA methyltransferases.
Structurally, DNA (cytosine-5)-methyltransferase 3A consists of an N-terminal regulatory region containing the PWWP and ADD domains, and a C-terminal catalytic domain with the conserved PCQ motif required for methyl group transfer. DNMT3A forms complexes with DNMT3L to enhance DNA binding and catalytic efficiency. This antibody enables investigation of DNMT3A's function in epigenetic gene silencing and oncogenic transformation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
