| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DOCK8 recombinant protein (Position: M1-S2099) was used as the immunogen for the DOCK8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DOCK8 Antibody / Dedicator of cytokinesis protein 8 is a anti-DOCK8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasm, cell membrane.
Key elements and design rationale
- Target: DOCK8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Dedicator of cytokinesis protein 8 contains a DOCK homology region (DHR) that mediates binding to CDC42 and phosphatidylinositol lipids. This structural configuration facilitates the localized activation of actin polymerization at the immunological synapse. The DOCK8 antibody enables visualization of this process, revealing how immune cells such as T cells, B cells, and natural killer cells use DOCK8 to maintain polarity and movement. Loss of function in DOCK8 disrupts actin organization, impairing immune synapse stability and antigen-specific responses.
Mutations in the DOCK8 gene cause autosomal recessive hyper-immunoglobulin E syndrome (HIES), characterized by recurrent viral infections, elevated IgE levels, and defective T and B cell function. The DOCK8 antibody supports studies into this condition by allowing detection of protein expression in immune cell subsets from affected patients. Functional loss of Dedicator of cytokinesis protein 8 leads to increased susceptibility to viral skin infections and defective immune surveillance, highlighting its critical role in immune defense.
Beyond immunodeficiency, Dedicator of cytokinesis protein 8 has been implicated in cancer biology, where its dysregulation can influence metastasis and tumor cell migration. The DOCK8 antibody supports investigations into these broader roles by enabling quantification of expression in cancer cell lines and tissues. Through regulation of actin assembly and cell adhesion, DOCK8 contributes to both immune and non-immune cell motility control.
The DOCK8 antibody performs effectively in western blotting, immunofluorescence, and flow cytometry, showing strong cytoplasmic localization consistent with its role in actin remodeling.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
