| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthetic peptide specific to Disruptor of telomeric silencing 1 like protein was used as the immunogen for the DOT1L antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DOT1L Antibody / KMT4 is a anti-DOT1L Rabbit antibody Recombinant Rabbit Monoclonal clone CBF-4 supplied in Purified format. Recommended for workflows such as Western blot (WB), IHC-P with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: DOT1L
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone CBF-4, isotype Rabbit IgG
- Format: Purified
- Applications (as listed): WB, IHC-P
Biological background
KMT4 functions by altering chromatin accessibility and creating binding sites for regulatory proteins that control transcription and DNA damage response. During development, DOT1L plays critical roles in embryogenesis, hematopoiesis, and differentiation. Loss of DOT1L disrupts stem cell renewal and lineage commitment, demonstrating its essential role in developmental biology. Its activity is also important for maintaining telomeric silencing and ensuring proper chromosomal function.
Aberrant DOT1L activity has been strongly implicated in cancer. In mixed lineage leukemia (MLL) rearranged leukemias, DOT1L is aberrantly recruited to oncogenic transcriptional complexes, leading to inappropriate H3K79 methylation and activation of leukemia promoting genes. This discovery has made DOT1L a therapeutic target, and small molecule inhibitors of DOT1L are in clinical trials for treatment of MLL associated leukemias. Beyond leukemia, dysregulated DOT1L activity has been observed in solid tumors, cardiovascular disease, and viral infection, underscoring its wide biological significance.
At the molecular level, DOT1L associates with transcriptional elongation complexes and other chromatin modifiers, linking histone modification with gene expression control. Structural studies show that DOT1L interacts directly with the nucleosome core, uniquely positioning it to catalyze methylation of H3K79. Through these mechanisms, DOT1L serves as an integrator of epigenetic signaling and transcriptional activity, shaping the cellular transcriptome in response to developmental and environmental signals.
The DOT1L antibody is commonly used in western blotting, immunohistochemistry, immunofluorescence, and flow cytometry to detect expression levels, nuclear localization, and disease associated changes. These applications are valuable for exploring transcriptional regulation, epigenetic therapy, and cancer biology. For researchers studying chromatin structure, RNA polymerase II elongation, or targeted therapeutics, the DOT1L antibody provides a reliable and specific detection tool.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
