DR3/NFκB Reporter Lentivirus

Background

DR3 (death receptor 3) is a member of the tumor necrosis factor receptor superfamily expressed predominantly on T lymphocytes and other immune cells. Its ligand, TL1A, engages DR3 to activate downstream signaling that can promote both T cell costimulation and, depending on context, pro-apoptotic responses. A major branch of DR3 signaling activates the NF-κB transcription factor, driving expression of genes involved in inflammation, immune cell activation, and survival. The TL1A-DR3 axis modulates T cell responses and is implicated in inflammatory and autoimmune diseases, making it an important target in immunology and immunotherapy research.

Product Description & Applications

The DR3/NFκB Reporter Lentivirus is an immunotherapy reporter system for studying DR3 receptor-mediated signaling. The construct constitutively expresses the DR3 receptor, which upon engagement by its ligand TL1A activates the NF-κB pathway, driving a dual reporter of secreted Gaussia luciferase and a fluorescent protein. This provides a quantitative readout of pro-apoptotic and inflammatory signaling and supports studies of receptor-engagement inhibition by blocking compounds or antibodies. Sequential transduction generates a stable reporter cell line, and secreted Gaussia luciferase enables non-destructive kinetic sampling from conditioned media. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, optimized for difficult-to-transfect cells, including primary and thawed cells.

About This Product

This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human DR3 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.

Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.