| Field | Specification |
|---|---|
| Mfr No | |
| Accession Number | |
| Product Type | |
| Reporter | |
| Selection Marker | Blasticidin, GFP (constitutively expressed), Hygromycin, Puromycin, RFP (constitutively expressed), Zeocin |
| Shipping | |
| Species |
Background
DR3 (death receptor 3) is a member of the tumor necrosis factor receptor superfamily expressed predominantly on T lymphocytes and other immune cells. Its ligand, TL1A, engages DR3 to activate downstream signaling that can promote both T cell costimulation and, depending on context, pro-apoptotic responses. A major branch of DR3 signaling activates the NF-κB transcription factor, driving expression of genes involved in inflammation, immune cell activation, and survival. The TL1A-DR3 axis modulates T cell responses and is implicated in inflammatory and autoimmune diseases, making it an important target in immunology and immunotherapy research.
Product Description & Applications
The DR3/NFκB Reporter Lentivirus is an immunotherapy reporter system for studying DR3 receptor-mediated signaling. The construct constitutively expresses the DR3 receptor, which upon engagement by its ligand TL1A activates the NF-κB pathway, driving a dual reporter of secreted Gaussia luciferase and a fluorescent protein. This provides a quantitative readout of pro-apoptotic and inflammatory signaling and supports studies of receptor-engagement inhibition by blocking compounds or antibodies. Sequential transduction generates a stable reporter cell line, and secreted Gaussia luciferase enables non-destructive kinetic sampling from conditioned media. Supplied as high-titer, VSV-G-pseudotyped third-generation lentiviral particles purified by PEG precipitation and sucrose gradient centrifugation, optimized for difficult-to-transfect cells, including primary and thawed cells.
About This Product
This 2-vial immunotherapy reporter system consists of a Vial 1 Receptor Lentivirus encoding human DR3 under a constitutive promoter with antibiotic selection, and a Vial 2 Reporter Lentivirus encoding tandem NFAT (or NF-κB) response elements driving a dual reporter (GFP, GFP-P2A-GLuc, GLuc, GLuc-P2A-GFP, GLuc-P2A-RFP, RFP, RFP-P2A-GLuc). Sequential transduction and selection generates a dual-stable effector cell line that responds quantitatively to receptor stimulation with a ratiometric fluorescent + bioluminescent readout.
Secreted Gaussia luciferase (where included) accumulates in conditioned media, enabling kinetic sampling without cell lysis. The combined fluorescent and luminescent outputs allow parallel microscopy-based visualization and plate-reader luminometry from the same cell population — providing assay redundancy and flexibility for potency testing formats compliant with regulatory expectations for cell-based functional assays.
Can't find the lentiviral construct you need, or want to adjust key design elements? Contact us to discuss custom LV design and optional add-ons.
Common customization requests
- Insert / payload: replace the gene/sequence, swap to a different isoform, add mutations, or optimize cloning features.
- Expression design: change promoter (e.g., CMV/EF1α/PGK), add enhancers, or adjust regulatory elements.
- Reporters: add/swap GFP/RFP/mCherry/luciferase (single or dual reporters where applicable).
- Selection markers: add/swap puromycin/blasticidin/neomycin or fluorescent selection options.
- Vector format: switch between OE, shRNA, CRISPR (sgRNA/Cas systems), or control vectors (where supported).
Add-ons you can request
- Control viruses: empty vector, non-targeting shRNA, reporter-only controls, or matched backbone controls.
- Packaging / format: concentration options, aliquoting, or custom fill volume for screening workflows.
- Documentation: construct map/sequence confirmation package (as available) and batch documentation.
What to include in your request
- Target cell type/model (cell line or primary cells) and intended readout (reporter, knockdown, OE, etc.)
- Insert sequence (FASTA) or reference ID, plus any required tags/mutations
- Promoter, reporter, and selection marker preferences
- Desired scale and preferred format (aliquots / concentration requests)
Email us at support@biohippo.com or use the Talk to a Scientist request form.
