| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human DTNB recombinant protein (Position: M1-G627) was used as the immunogen for the DTNB antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DTNB Antibody / Dystrobrevin beta is a anti-DTNB Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DTNB
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
DTNB is encoded by the DTNB gene located on human chromosome 2p16.2. The protein is approximately 628 amino acids in length and shares structural similarity with dystrobrevin alpha. DTNB localizes to the cytoplasmic face of the sarcolemma and interacts with dystrophin, syntrophins, and other adaptor proteins within the dystrophin-glycoprotein complex (DGC), which links the cytoskeleton to the extracellular matrix.
The DTNB antibody detects a 70 kilodalton protein by western blot and reveals sarcolemmal and cytoplasmic localization under immunofluorescence microscopy. In skeletal muscle, DTNB contributes to stabilization of muscle fibers and transmission of mechanical force. In neurons, it participates in postsynaptic density organization and signal transduction, influencing synaptic plasticity and neurotransmission.
Dysfunction or loss of DTNB disrupts DGC integrity, leading to impaired muscle contractility and signaling abnormalities. Variants in DTNB have been associated with neuromuscular diseases, cognitive deficits, and cardiomyopathy. Through its scaffolding functions, DTNB regulates localization of signaling molecules such as nitric oxide synthase and kinases involved in muscle adaptation.
Because of its role in linking the cytoskeleton to membrane signaling complexes, DTNB provides insights into muscular dystrophy mechanisms and synaptic organization.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
