| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human Dysbindin/DTNBP1 recombinant protein (Position: M1-S351) was used as the immunogen for the DTNBP1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DTNBP1 Antibody / Dysbindin is a anti-DTNBP1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: DTNBP1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
Dysbindin interacts with other BLOC-1 subunits, including SNAPIN, MUTED, and BLOC1S proteins, coordinating actin-based vesicular transport. In neurons, it contributes to synaptic vesicle recycling and glutamatergic signaling, influencing synaptic plasticity and cognitive processes. Dysbindin localizes to endosomes and synaptic vesicles, reflecting its role in membrane trafficking and receptor recycling.
The DTNBP1 antibody is widely used in neuroscience, cell biology, and genetic disease research to examine endosomal transport, synaptic vesicle dynamics, and intracellular trafficking. Western blot analysis identifies a 40 kilodalton band corresponding to Dysbindin, while immunofluorescence shows punctate cytoplasmic staining consistent with endosomal localization. This antibody supports investigations into molecular mechanisms of vesicle regulation and neurotransmission.
Loss-of-function mutations or reduced expression of DTNBP1 have been associated with Hermansky-Pudlak syndrome type 7 and susceptibility to schizophrenia, linking Dysbindin to synaptic homeostasis and neurodevelopment. Dysbindin also influences muscle membrane stability by binding to dystrobrevins. The DTNBP1 antibody provides a dependable reagent for exploring vesicle trafficking, neurological function, and genetic disorders.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
