| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human TCTEX1 was used as the immunogen for the DYNLT1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
DYNLT1 Antibody / TCTEX1 is a anti-DYNLT1 Rabbit antibody Recombinant Rabbit Monoclonal clone 30D84 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human, Mouse.
Key elements and design rationale
- Target: DYNLT1
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30D84, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
DYNLT1 antibody is widely used in cell biology, neuroscience, and developmental research. In neurons, DYNLT1 mediates retrograde transport of signaling endosomes, mitochondria, and synaptic vesicles. This process is essential for synaptic maintenance, neuronal survival, and communication between axons and cell bodies. Disruption of DYNLT1 impairs axonal transport and has been linked to neurodevelopmental and neurodegenerative diseases. By detecting DYNLT1, researchers can evaluate the molecular basis of dynein cargo interactions and their impact on neuronal function.
Western blot assays reveal DYNLT1 protein bands in cell lysates and neuronal extracts. Immunohistochemistry maps DYNLT1 expression in brain and spinal cord tissues, while immunofluorescence highlights punctate localization consistent with vesicular transport complexes. These methods make DYNLT1 antibody a versatile tool for analyzing retrograde trafficking pathways.
DYNLT1 also plays roles in viral infection, where it is hijacked by pathogens to facilitate retrograde transport of viral components. Additionally, DYNLT1 has been implicated in mitotic spindle positioning, apoptosis regulation, and intracellular signaling. By applying DYNLT1 antibody, scientists can investigate how dynein light chain proteins integrate diverse cellular processes through transport regulation.
DYNLT1 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
