| Field | Specification |
|---|---|
| Mfr No | |
| Expression System | |
| Form | Liquid |
| Product Type | |
| Reporter | |
| Storage | |
| Target |
Scientific Background
Zaire Ebola virus (EBOV) is the most lethal species of the Ebolavirus genus, responsible for multiple large outbreaks including the 2014–2016 West Africa epidemic. EBOV is classified as a BSL-4 pathogen, with case fatality rates of up to 90% in untreated patients. The viral glycoprotein (GP) mediates cell entry via macropinocytosis and NPC1-dependent membrane fusion. Safe surrogate systems for studying EBOV entry and evaluating anti-EBOV countermeasures are critical for biodefense and therapeutic development.
Product Description
Filovirus
This HA-EBOV VLP is a hybrid alpha-pseudovirus assembled from Ebola Virus Zaire structural proteins (GP, VP40, NP) with an alphavirus-derived reporter RNA payload. BSL-2 safe and ready to use for studying viral entry. Available with Firefly luciferase, GFP, or RFP reporter.
Product Specifications
| Catalog Number | 0571-PP-001 |
|---|---|
| Virus Strain | Zaire |
| Structural Proteins | GP (glycoprotein), VP40 (matrix protein), NP (nucleoprotein) |
| Reporter Payload | Alphavirus-derived self-replicating RNA encoding Firefly Luciferase, GFP, or RFP |
| Particle Type | Single-cycle alpha-pseudovirus (BSL-2 safe) |
| Form | Liquid |
| Size | 1X Concentrated: 25 × 200 µL (~100 wells per 96-well plate) |
| Storage | -80°C. Avoid repeated freeze-thaw cycles. |
Applications
- Ebola pseudovirus transduction of target cells for viral entry and functional studies
- Rapid anti-Ebola drug and entry inhibitor screening
- Rapid anti-Ebola neutralizing antibody screening and IC50 determination
Safety & Handling
Yes. HA-EBOV VLPs are single-cycle pseudoviruses that cannot produce infectious Ebola virus. They can be handled under standard BSL-2 containment, enabling entry and neutralization studies without BSL-4 requirements.
Vero or other permissive cells are infected with HA-EBOV VLPs or a VLP/test-sample mixture for ~1 hr. After overnight incubation, reporter expression (luciferase or fluorescent protein) is quantified. Neutralization is measured as percentage inhibition relative to virus-only controls.
VeroE6 cells are commonly used for filovirus pseudovirus assays. HEK293T cells expressing the NPC1 receptor can also be used for enhanced transduction efficiency in some protocols.
Need a different reporter, pack size, or bulk volume? BioHippo works directly with IBT Bioservices to support custom and large-volume requests. Contact us for a quote or to discuss your project requirements.
Looking for matching antigen proteins, antibodies, or other coronavirus/filovirus research reagents? Contact BioHippo support for recommendations.
