| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human EDEM1 recombinant protein (Position: P124-I657) was used as the immunogen for the EDEM1 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EDEM1 Antibody / ER degradation-enhancing alpha-mannosidase-like protein 1 is a anti-EDEM1 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: EDEM1
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
EDEM1 is encoded by the EDEM1 gene located on human chromosome 3p26.1. The protein is approximately 766 amino acids in length and belongs to the glycosyl hydrolase 47 family. EDEM1 localizes to the lumen of the endoplasmic reticulum, where it associates with chaperones such as calnexin and BiP. It functions by accelerating the removal of mannose residues from misfolded glycoproteins, signaling their export from the ER for proteasomal degradation.
The EDEM1 antibody detects an 85 kilodalton band in western blot assays and exhibits reticular ER staining under confocal microscopy. EDEM1 plays a pivotal role in maintaining proteostasis by preventing accumulation of misfolded proteins, thereby protecting cells from ER stress-induced apoptosis. Under stress conditions, expression of EDEM1 is upregulated through the unfolded protein response (UPR), particularly by transcription factors ATF6 and XBP1.
Dysregulation of EDEM1 contributes to neurodegenerative diseases, diabetes, and cancer, where chronic ER stress leads to cell dysfunction. In addition to its role in degradation, EDEM1 fine-tunes glycoprotein folding efficiency and influences secretion rates of correctly folded proteins. Because EDEM1 acts upstream of ERAD, it serves as an early determinant of glycoprotein fate within the secretory pathway.
Through its key role in ER quality control, EDEM1 ensures fidelity of protein folding and cellular homeostasis.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
