EFNA1 Antibody / Ephrin A1

Overview

EFNA1 Antibody / Ephrin A1 is an antibody targeting EFNA1, raised in Mouse for protein detection and localization studies where these specifications are required.

Key elements and design rationale

• Target: EFNA1.
• Antibody identity: Polyclonal (rabbit origin); Rabbit Ig.
• Conjugate/label: Unconjugated (affects detection chemistry and multiplex compatibility).
• Format: Purified.
• Species reactivity: Human.
• Listed applications: WB, IF (refer to on-page specifications for application-specific guidance).

Biological background

Ephrin A1 is a membrane-bound protein that interacts with Eph receptors on neighboring cells, initiating a cascade of signals that regulate cell behavior and movement. This interaction is essential for processes such as cell migration, tissue organization, and synaptic connectivity in the nervous system. Studies have shown that dysregulation of Ephrin A1 signaling can lead to aberrant cell growth, invasion, and metastasis in cancer cells, highlighting its importance in disease progression. One of the key features of Ephrin A1 is its ability to both attract and repel cells, depending on the context and concentration of the molecule. This dual role allows Ephrin A1 to fine-tune cell-cell interactions and ensure proper tissue patterning during development. In the brain, for example, Ephrin A1 guides axonal growth and synapse formation, shaping the intricate neural circuits that underlie cognitive functions. In addition to its role in development, Ephrin A1 has also been implicated in several disease processes. Aberrant expression of EFNA1 has been observed in various types of cancer, including breast, lung, and colon cancer, where it promotes tumor growth and invasion. In neurodegenerative disorders such as Alzheimer's disease, dysregulation of Ephrin A1 signaling disrupts synaptic connectivity and contributes to cognitive decline.

Research relevance and current trends

• Comparative expression profiling across cell types, tissues, or perturbations (e.g., drug treatment, genetic editing, or differentiation).
• Subcellular localization and trafficking studies, including co-localization with pathway markers in microscopy-based assays.
• Integration of protein-level measurements with transcriptomics or proteomics to relate abundance to regulation and phenotype.

Common research applications

• Western blotting: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.
• Immunofluorescence: researchers commonly compare relative signal levels across conditions and use appropriate negative/positive controls for interpretation.

Interpretation should account for antibody-dependent factors such as epitope accessibility, isoforms, and sample preparation differences across workflows.

Notes for experimental interpretation

• Isoforms and PTMs: many targets have multiple isoforms and post-translational modifications that can shift apparent signal or localization; interpret bands/signals accordingly.
• Epitope context: binding can depend on protein conformation and sample processing; region information in the title/immunogen can help anticipate what may be detected.
• Species differences: predicted or validated reactivity may vary by ortholog sequence and sample context; confirm in your model system.
• Control concepts: include negative controls (no-primary/isotype), and where possible genetic controls (KO/KD) or independent antibodies to strengthen conclusions.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.