| Field | Specification |
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Scientific Background
The EGFR (epidermal growth factor receptor), also known as ErbB-1 and HER1, is a cell surface tyrosine kinase receptor belonging to the epidermal growth factor receptor family. Overexpression and/or hyperactivation of EGFR kinase is associated with several human cancers such as lung, glioblastoma, and epithelial tumors of the neck and head. This increased activity of EGFR has led to the development of anticancer therapeutics targeting EGFR. However, d746-750, T790M, and C797S mutations in EGFR can cause resistance to known EGFR inhibitors, requiring the development of next generation mutant-selective inhibitors.
Product Description
Recombinant ProteinRecombinant EGFR (T790M protein is produced using a validated recombinant expression system and supplied in aqueous buffer solution. Suitable for enzyme kinetics, inhibitor screening, binding assays, structural studies, and related biochemical research applications.
Protein Specifications
| UniProt ID | P00533 |
|---|---|
| Storage | This assay kit will perform optimally for up to 6 months from date of receipt wh… |
| Biosafety Level | Not applicable (BSL-1) |
Safety & Handling
Avoid freeze/thaw cycles.
This protein was produced in a recombinant expression system. Expression system selection determines post-translational processing, disulfide bond formation, and co-factor incorporation — all of which affect enzymatic activity. Insect cell (Sf9) systems are preferred for kinases and multi-subunit enzymes that require phosphorylation or chaperone assistance; E. coli is used for structurally simpler proteins.
The expressed amino acid region is specified in the product description. Confirm the region includes your domain of interest — the active site, binding pocket, or substrate recognition sequence — before placing your order. Refer to the UniProt database for domain annotation.
Purity is assessed by SDS-PAGE; see the Certificate of Analysis. A gel image is provided with each lot. BPS Bioscience performs rigorous QC on each lot, including purity assessment and functional activity testing where applicable. Contact technical support if purity ≥99% is required for biophysical measurements.
Study enzyme kinetics and screen small molecular inhibitors for drug discovery and high throughput (HTS) applications. Refer to the product datasheet for validated protocols and recommended assay conditions. Contact BioHippo technical support for application-specific guidance.
This assay kit will perform optimally for up to 6 months from date of receipt when the materials are stored as directed. Avoid repeated freeze-thaw cycles — prepare single-use working aliquots. Add BSA or glycerol to aliquots if storing diluted enzyme is necessary. Typical stability is at least 6 months at −80°C.
BioHippo offers flexible sourcing for qualified research institutions and partners.
- Bulk quantities: Large-scale orders for HTS campaigns or structural studies.
- Custom constructs: Alternative tag positions, truncation variants, or point mutants may be available upon request.
- Biotinylated variants: Avi-Tag site-specific biotinylation is available for SPR/BLI surface capture applications.
- Extended QC data: Activity assay data, SEC-HPLC profiles, or additional purity methods available on request.
Contact BioHippo customer support for custom requirements.
