| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human EGR2 recombinant protein (Position: M1-R257) was used as the immunogen for the EGR2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EGR2 Antibody / Early growth response protein 2 is a anti-EGR2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: EGR2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
During development, EGR2 plays a critical role in establishing segmental identity within the hindbrain by defining rhombomere boundaries and activating patterning genes. In Schwann cells, EGR2 drives transcription of myelin structural proteins including MPZ, PMP22, and MBP, ensuring proper myelin sheath formation and maintenance. Mutations in EGR2 cause hereditary neuropathies such as Charcot-Marie-Tooth disease type 1D and Dejerine-Sottas syndrome, underscoring its indispensable function in myelination. The protein also participates in T-cell anergy and macrophage activation by regulating cytokine gene expression.
The EGR2 antibody is widely used in neurobiology, developmental biology, and immunology to detect EGR2 protein expression and localization. Western blot analysis typically shows a 50 kilodalton band, while immunohistochemistry reveals strong nuclear staining in Schwann cells and developing neurons. Expression of EGR2 is inducible by growth factors, nerve injury, and mechanical stress, making it a sensitive marker for differentiation and regeneration. In the immune system, EGR2 acts as a transcriptional repressor in T cells to maintain peripheral tolerance by suppressing activation genes.
Beyond its physiological roles, EGR2 has been implicated in tumor biology and inflammatory disease. Altered EGR2 signaling can disrupt differentiation programs and contribute to leukemogenesis or autoimmune disorders. In cancer models, EGR2 functions context-dependently as either a tumor suppressor or pro-survival factor, depending on cellular environment. The EGR2 antibody allows researchers to explore these regulatory networks, providing a means to monitor transcriptional changes during neural development and immune modulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
