| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human eIF1A was used as the immunogen for the EIF1AX antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EIF1AX Antibody / eIF1A X isoform is a anti-EIF1AX Rabbit antibody Recombinant Rabbit Monoclonal clone 29E78 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS) with listed reactivity in Human, Mouse, Rat.
Key elements and design rationale
- Target: EIF1AX
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29E78, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP, FACS
Biological background
EIF1AX antibody is widely applied in molecular biology, cancer research, and developmental studies. Translation initiation is a highly regulated step in gene expression, and EIF1AX ensures fidelity in AUG codon selection. Mutations in EIF1AX have been identified in uveal melanoma, thyroid cancer, and endometrial carcinoma, where they cooperate with other oncogenic events. By detecting EIF1AX, researchers can study how altered initiation contributes to tumor biology and translational control.
Applications for EIF1AX antibody include western blotting, immunohistochemistry, and immunofluorescence. Western blotting detects EIF1AX expression in cell lysates, immunohistochemistry maps its expression in tumors and tissues, and immunofluorescence highlights nuclear and cytoplasmic localization. These assays provide strong tools to evaluate translation initiation factor biology in both normal and disease contexts.
EIF1AX mutations often cluster at the N terminus, affecting interactions with other initiation factors and altering start codon selection. Dysregulation of initiation leads to abnormal gene expression patterns, contributing to cell proliferation and tumorigenesis. By applying EIF1AX antibody, scientists can study these processes in detail and evaluate EIF1AX as a potential biomarker or therapeutic target.
EIF1AX also functions in normal development, where accurate translation initiation is critical for embryogenesis and tissue growth. Its conservation across species highlights its fundamental role in biology. The antibody therefore supports research from basic ribosome biology to applied cancer translational studies.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
