| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | A synthesized peptide derived from human eIF1A was used as the immunogen for the eIF1A antibody / EIF1AX. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EIF1AX Antibody / eIF1A X isoform is a anti-EIF1AX Rabbit antibody Recombinant Rabbit Monoclonal clone 29E67 supplied in Liquid format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP) with listed reactivity in Human.
Key elements and design rationale
- Target: EIF1AX
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 29E67, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB, IHC, ICC, IF, IP
Biological background
Eukaryotic translation initiation factor 1A X linked is a member of the EIF1A family, which includes autosomal EIF1A and the X chromosome encoded isoform. Although both proteins share significant homology and overlapping function, EIF1AX has unique tissue expression and regulatory features. Research using EIF1AX antibody has demonstrated that depletion of this factor disrupts translation initiation, leading to defective ribosome loading and reduced protein synthesis. This highlights its central role in the earliest step of gene expression control.
Mutations in EIF1AX have been identified in several cancers, including uveal melanoma, thyroid carcinoma, and ovarian cancer. These mutations cluster in the N terminal tail of the protein and are thought to alter ribosomal scanning or initiation fidelity. Studies with EIF1AX antibody have shown that mutated EIF1AX expression alters global translation profiles, potentially driving oncogenesis by enhancing the synthesis of pro survival or proliferative proteins. This makes EIF1AX both a cancer biomarker and a potential therapeutic target.
Beyond cancer, EIF1AX is essential for development and cellular homeostasis. Research using EIF1AX antibody has revealed that knockdown in model organisms causes embryonic lethality due to failure of translation initiation. Its expression is enriched in proliferative tissues, consistent with high protein synthesis demands. Because translation initiation is tightly linked to growth signals such as mTOR and MAPK pathways, EIF1AX occupies a central position in connecting signaling to protein production.
EIF1AX antibody is used in western blotting, immunohistochemistry, and immunofluorescence. Western blotting confirms protein expression across proliferative cell lines, immunohistochemistry localizes EIF1AX in tissue sections, and immunofluorescence highlights its presence in the cytoplasm and nucleus. These techniques allow researchers to assess expression changes, localization, and associations with ribosomal components. Functional studies with EIF1AX antibody include co immunoprecipitation, revealing its interactions with initiation complex proteins and ribosomal subunits.
By supplying validated EIF1AX antibody reagents,
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
