| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human EIF2A recombinant protein (Position: H24-I585) was used as the immunogen for the EIF2A antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EIF2A Antibody / Eukaryotic translation initiation factor 2A is a anti-EIF2A Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Rat. Reported localization: Cytoplasm.
Key elements and design rationale
- Target: EIF2A
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, EIF2A antibody recognizes a 585-amino-acid cytoplasmic protein that mediates the recruitment of initiator Met-tRNA to the ribosome in an EIF2-independent manner. EIF2A interacts with EIF3, ribosomal subunits, and tRNA to enable translation initiation, particularly for internal ribosome entry site (IRES)-containing mRNAs or stress-induced transcripts. Its activity contrasts with EIF2S1 (EIF2-alpha), which forms the classic EIF2 complex with GTP and initiator tRNA.
The EIF2A gene is located on chromosome 3q26.2 and is ubiquitously expressed, with high levels in pancreas, liver, and testis. During cellular stress, EIF2A supports translation of mRNAs involved in survival and recovery, including those that escape EIF2-alpha phosphorylation-mediated repression. It also contributes to viral mRNA translation, highlighting its role in host-pathogen interactions.
Pathologically, altered EIF2A function has been linked to metabolic disorders, neurodegenerative diseases, and cancer. Its role in selective translation during stress responses connects it to the unfolded protein response and integrated stress response pathways. Research with EIF2A antibody enables detection of translational shifts and analysis of noncanonical initiation mechanisms.
EIF2A antibody is suitable for western blot, immunocytochemistry, and immunoprecipitation to detect EIF2A in cultured cells and tissues. It is commonly used in studies of translational control, stress granule dynamics, and viral infection models.
Structurally, EIF2A is composed of N-terminal domains that interact with tRNA and ribosomal proteins, and C-terminal extensions involved in EIF3 binding. It differs mechanistically from EIF2S1 in GTP usage and codon selection. This antibody helps delineate EIF2A's contribution to alternative initiation and translational adaptation under stress conditions.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
