| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human EIF3H recombinant protein (Position: K37-N352) was used as the immunogen for the EIF3H antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EIF3H Antibody / Eukaryotic translation initiation factor 3 subunit H is a anti-EIF3H Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Cytoplasmic.
Key elements and design rationale
- Target: EIF3H
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, FACS, ELISA
Biological background
Functionally, Eukaryotic translation initiation factor 3 subunit H supports assembly of the 43S pre-initiation complex and helps maintain interactions with eIF4F-bound mRNAs. Perturbations in subunit stoichiometry can shift translation of specific mRNA cohorts, including transcripts with structured 5' UTRs or upstream open reading frames. In cancer models, amplification or overexpression of Eukaryotic translation initiation factor 3 subunit H correlates with elevated global translation and selective enhancement of growth-promoting messages. These properties make EIF3H Antibody valuable for charting how initiation control intersects with oncogenic signaling and stress adaptation.
In the laboratory, EIF3H Antibody enables detection of the native subunit in immunoblotting, monitors changes during serum starvation or mTOR pathway modulation, and supports co-immunoprecipitation to define interactions within the eIF3 complex. In immunofluorescence, the antibody reveals cytoplasmic distribution patterns that can be aligned with ribosomal markers or stress granule components. Researchers coupling EIF3H Antibody with polysome profiling, ribosome footprinting, or cap-binding assays can connect subunit abundance to translational throughput and transcript selectivity. Because initiation control influences cell growth, differentiation, and stress resilience, accurate measurement of this subunit helps interpret the translational state of diverse cell types.
The EIF3H Antibody from
As translation control emerges as a central driver of cell behavior, EIF3H Antibody provides a dependable means to quantify and localize a key eIF3 subunit, enabling researchers to connect molecular initiation events to system-level outcomes in development, cancer, and stress biology.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
