| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human EIF4A3 recombinant protein (Position: R14-I411) was used as the immunogen for the EIF4A3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EIF4A3 Antibody / Eukaryotic initiation factor 4A-III is a anti-EIF4A3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear, cytoplasmic.
Key elements and design rationale
- Target: EIF4A3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, ICC/IF, IP, FACS, ELISA
Biological background
EIF4A3 functions as an mRNA quality control factor by binding to spliced mRNAs upstream of exon-exon junctions as part of the EJC, together with MAGOH, RBM8A (Y14), and MLN51. This complex regulates nonsense-mediated mRNA decay and translation efficiency, ensuring that defective transcripts are degraded. EIF4A3 also acts independently as an RNA helicase that unwinds RNA secondary structures to facilitate ribosome scanning during translation initiation. Co-localization studies show EIF4A3 concentrated in nuclear speckles and cytoplasmic ribonucleoprotein granules, reflecting its dual role in RNA processing and translation.
Structurally, EIF4A3 contains two RecA-like domains forming an ATP-binding cleft and conserved motifs such as the DEAD (Asp-Glu-Ala-Asp) box essential for helicase activity. It belongs to the DEAD-box helicase subfamily of eukaryotic initiation factors, which also includes EIF4A1 and EIF4A2. Unlike these cytoplasmic isoforms, EIF4A3 primarily functions in the nucleus as part of the EJC, anchoring the complex to mRNA. Known interacting partners include MAGOH, Y14, UPF1, and CASC3.
Functionally, EIF4A3 is indispensable for mRNA stability, nonsense-mediated decay, and post-transcriptional gene regulation. It coordinates RNA splicing and surveillance with translation, maintaining proteome integrity. In neurons, EIF4A3 regulates synaptic mRNA localization and local protein synthesis critical for plasticity. During embryonic development, EIF4A3 contributes to neural differentiation and morphogenesis by regulating gene expression at the RNA level.
Mutations or depletion of EIF4A3 disrupts RNA surveillance, leading to aberrant transcript accumulation and developmental defects. Loss-of-function variants cause Richieri-Costa-Pereira syndrome, characterized by craniofacial and limb malformations. Dysregulation of EIF4A3 expression has also been associated with tumor progression, as enhanced RNA surveillance supports oncogenic growth. Pathway associations include mRNA splicing, nonsense-mediated decay, and translational control.
Immunohistochemical staining using EIF4A3 antibody demonstrates nuclear and cytoplasmic localization in neurons, epithelial cells, and germ cells. The EIF4A3 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
