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Product Overview
CD80 is a 60 kD highly glycosylated protein. It is a member of the Ig superfamily and is also known as B7-1, B7, and Ly-53. CD80 is constitutively expressed on dendritic cells and monocytes/macrophages, and inducibly expressed on activated B and T cells. The ligation of CD28 on T cells with CD80 and CD86 (B7-2) on antigen presenting cells (such as dendritic cells, macrophages, and B cells) elicits co-stimulation of T cells resulting in enhanced cell activation, proliferation, and cytokine production. CD80 appears to be expressed later in the immune response than CD86. CD80 can also bind to CD152, also known as CTLA-4, to deliver an inhibitory signal to T cells.
Validated Applications & Recommended Dilutions
| Application | Recommended Dilution |
|---|---|
| Flow Cytometry | See product datasheet |
Antibody Specifications
| Target | CD80 |
|---|---|
| Host Species | Armenian Hamster |
| Clonality | Monoclonal |
| Isotype | Armenian Hamster IgG |
| Conjugate | Elab Fluor®Red 780 |
| Clone Number | 16-10A1 |
| Purification | See datasheet |
| Concentration | 5 μL/Test |
Immunogen
Recombinant protein fragment; see full datasheet. UniProt: Q00609.
Species Reactivity & Cross-Reactivity
Validated for: Mouse. Verify suitability in your sample type prior to use. For species or applications not listed in the datasheet, consult our technical team before purchase.
Storage & Handling
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze.
Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.
Related Products
Browse additional antibodies targeting CD80 and complementary reagents — including secondary antibodies, blocking buffers, and detection kits — available through BioHippo.
This antibody is validated for flow cytometry applications. For intracellular targets, fix and permeabilize cells using a validated fixation/permeabilization kit (e.g., BD Cytofix/Cytoperm or similar) before staining. For surface markers, stain live or fixed cells without permeabilization. Always include a matched isotype control (Armenian Hamster IgG, Armenian Hamster origin) at the same protein concentration as the test antibody to establish non-specific binding thresholds. Recommended starting dilution/concentration: see datasheet.
This antibody has been experimentally validated in: Mouse. Cross-reactivity with other species has not been systematically evaluated. If your sample species is not listed, assess immunogen sequence homology to your target species ortholog. The immunogen used is: "" — higher sequence identity between species increases the probability of cross-reactivity, but experimental validation with appropriate controls is necessary before drawing scientific conclusions.
This product can be stored at 2-8°C for 12 months. Please protected from prolonged exposure to light and do not freeze. Storage Buffer: Phosphate buffered solution, pH 7.2, containing 0.09% stabilizer and 1% protein protectant.. To maintain antibody activity, avoid repeated freeze-thaw cycles. Aliquot into single-use volumes before first freeze. If working with the antibody frequently, keep a working stock at 4°C for up to 4 weeks — add 0.02% sodium azide to the working stock as a bacteriostatic agent if azide is not already present in the buffer. Monitor performance against a validated positive control when transitioning between storage aliquots or lots.
This monoclonal antibody (clone 16-10A1) provides highly consistent, epitope-specific recognition of CD80, making it ideal for quantitative assays, multiplex panels, and studies requiring strict reproducibility across experiments and independent laboratories. Unlike polyclonal preparations, each production batch from a single hybridoma clone generates antibody with identical epitope specificity, affinity, and isotype — ensuring reliable signal comparison in longitudinal studies or multi-site collaborations. When citing this antibody in publications, include the clone designation (clone 16-10A1) to enable third-party reproducibility verification.
Customization & Add-ons: Can't find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
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