| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ELF3 recombinant protein (Position: Q48-L314) was used as the immunogen for the ELF3 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ELF3 Antibody / E74-like factor 3 is a anti-ELF3 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunoprecipitation (IP), ELISA with listed reactivity in Human.
Key elements and design rationale
- Target: ELF3
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IP, ELISA
Biological background
E74-like factor 3 contains a highly conserved ETS DNA-binding domain and transcriptional activation region that interact with coactivators such as CBP/p300. It is predominantly expressed in epithelial tissues including the gastrointestinal tract, lung, and mammary gland. The ELF3 antibody enables visualization of nuclear expression patterns in these tissues and supports mechanistic studies of epithelial-specific gene networks. In normal physiology, ELF3 regulates differentiation of epithelial cells by coordinating transcription of structural and signaling molecules necessary for tissue integrity.
Aberrant expression of ELF3 has been linked to carcinogenesis, especially in colorectal, breast, and bladder cancers. It may function as either a tumor suppressor or oncogene depending on context, influencing cell proliferation, invasion, and response to inflammatory cytokines. The ELF3 antibody allows quantitative and localization-based studies of this transcription factor in tumor samples, contributing to identification of prognostic markers and molecular subtypes. It also facilitates exploration of ELF3's interaction with beta-catenin and NF-?B pathways, which connect epithelial transcription programs to inflammation and cancer progression.
In immune regulation, E74-like factor 3 drives transcription of chemokines and adhesion molecules that coordinate epithelial immune responses to pathogens and injury. The ELF3 antibody supports studies into these processes, revealing how epithelial tissues integrate immune and barrier functions. Furthermore, ELF3 participates in developmental processes such as endoderm differentiation and organ morphogenesis by directing epithelial lineage specification. Its dysregulation in early development may contribute to congenital anomalies and tissue remodeling defects.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
