| Field | Specification |
|---|---|
| Mfr No | |
| Alternative Names | Glucagon Like Peptide 1; GLP-1; GLP1; GLP 1 |
| Assay Time | |
| Assay Type | |
| Detection Method | |
| Product Type | |
| Reactivity | |
| Sample Type(s) | Serum, Plasma, Cell culture supernatants, Cell lysates, and cell culture supernatant |
| Shipping | |
| Storage |
Features & Benefits
EliKine™ Rat GLP-1 Competitive ELISA Kit has high sensitivity and excellent specificity for detection of Rat GLP-1.
Background
GLP-1 is a peptide hormone released from intestinal L-cells upon nutrient consumption. It bindsthe GLP-1 receptorin the pancreas and displays various antidiabetic effects by potentiating glucose-induced secretion of insulin from pancreatic β-cells, increasing insulin expression. EliKine™ Mouse GLP-1 Competitive ELISA Kit uses a competitive method to quantify mouse GLP-1 in a sample. Mouse specific antibody against glucagon-like peptide 1(GLP-1) was coated on the enzyme label plate, and the antigen to be tested (sample or standard) and biotin-labeled antigen were added to the well of the enzyme label plate at the same time. The antigen to be tested and the biotin-labeled antigen were competitively bound to the specific antibody. After washing, the proprietary streptavidin-HRP conjugate was added to the wells. After washing and removing any unbound streptavidin-HRP, adding HRP Substrate (TMB), TMB turns blue under the catalysis of HRP, and turns yellow after adding stop solution. Measure the OD value with a microplate reader at 450 nm wavelength. The Mouse GLP-1 concentration is inversely proportional to the OD450 nm value.
This Rat GLP-1 Competitive ELISA kit is validated for use with Serum, Plasma, Cell culture supernatants, Cell lysates, and cell culture supernatant. Samples should be collected, processed, and stored correctly to preserve analyte integrity — avoid repeated freeze-thaw cycles and centrifuge to remove particulates before use. Dilute samples exceeding the kit's detection range using the supplied assay diluent. Hemolytic, icteric, or lipemic samples may affect assay performance and should be tested with caution.
This is a competitive ELISA kit employing an HRP (horseradish peroxidase)-conjugated secondary antibody paired with a TMB (3,3′,5,5′-tetramethylbenzidine) colorimetric substrate. In the competitive format, the target analyte Rat GLP-1 Competitive captured on the microplate surface is detected by the conjugated antibody, generating a colorimetric signal proportional to analyte concentration. The reaction is stopped and absorbance measured at 450 nm on a standard microplate reader.
The complete protocol, from sample addition to final plate reading, requires approximately 3–5 hours. This includes two incubation periods (analyte binding and detection antibody steps), intermediate wash cycles to remove unbound material, 15–30 minutes of TMB substrate development, and final stop-solution addition before absorbance reading. Exact timing will vary with experience level and the number of samples processed in parallel.
Required equipment: (1) a microplate spectrophotometer capable of reading absorbance at 450 nm (reference wavelength 540–570 nm recommended for background correction); (2) precision single-channel or multichannel pipettes; (3) a plate washer or multichannel aspirator; (4) a microcentrifuge for sample clarification; and (5) a 37°C incubator or stable room-temperature environment. No fluorescence or luminescence reader is required — standard colorimetric plate readers are fully compatible with this kit.
This ELISA kit is formally validated for Rat. Cross-reactivity with species not listed in the specification has not been independently characterized. Variability in protein sequence homology across species means that performance in unlisted species cannot be guaranteed without additional validation. For cross-species detection requirements or non-standard sample matrices, please contact BioHippo support or refer to the manufacturer's technical team for guidance.
Can't Find What You're Looking For? We can help you source the best match or customize an ELISA solution for your study. Options may include alternative target synonyms, different species reactivity, sample type/matrix compatibility (serum/plasma/lysate/supernatant), assay format (sandwich/competitive), sensitivity/range, detection chemistry (colorimetric/fluorescent/chemiluminescent), plate format (pre-coated/uncoated, strips vs full plate), and bulk or custom packaging. Click Talk to a Scientist to submit a request form, email us at support@biohippo.com, or explore our Research Services for additional support. Our team will be in contact with you shortly.
