| Field | Specification |
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| Immunogen | A synthesized peptide derived from human TCEB2 was used as the immunogen for the ELOB antibody. |
| Isotype | |
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| Reactivity | |
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| Target | |
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Overview
ELOB Antibody / Elongin B is a anti-ELOB Rabbit antibody Recombinant Rabbit Monoclonal clone 30E89 supplied in Liquid format. Recommended for workflows such as Western blot (WB) with listed reactivity in Human.
Key elements and design rationale
- Target: ELOB
- Antibody details: Rabbit, Recombinant Rabbit Monoclonal, clone 30E89, isotype Rabbit IgG
- Format: Liquid
- Applications (as listed): WB
Biological background
ELOB antibody is widely applied in transcription research, ubiquitin signaling, and cancer biology. As part of the elongin complex, elongin B facilitates efficient transcription of protein coding genes. In addition, elongin B interacts with Cullin 2 and von Hippel Lindau tumor suppressor protein to form an E3 ubiquitin ligase complex, which targets hypoxia inducible factor alpha for degradation. By detecting ELOB, researchers can study both transcriptional regulation and proteostasis.
Western blotting with ELOB antibody detects protein expression in nuclear extracts. Immunohistochemistry maps ELOB distribution in proliferative tissues, while immunofluorescence shows localization in the nucleus and cytoplasm. These assays make ELOB antibody a versatile tool for understanding transcription and protein turnover.
ELOB plays critical roles in hypoxia signaling. The elongin complex with von Hippel Lindau protein mediates degradation of HIF1 alpha under normoxia, maintaining cellular oxygen sensing. Dysregulation of this pathway contributes to tumorigenesis and adaptation to hypoxia. By applying ELOB antibody, scientists can investigate how ubiquitin ligase complexes control oxygen sensing and cancer biology.
Beyond hypoxia signaling, elongin B supports general protein degradation pathways, acting as a ubiquitin like protein that enhances complex stability. It also contributes to viral replication, as some viruses hijack elongin containing complexes for their own protein turnover. The antibody thus supports studies in virology as well as cell biology.
Mutations or dysregulation of ELOB are linked to cancer progression and altered stress responses. Because of its role in both transcription and protein degradation, elongin B integrates signaling networks that control cell survival and growth. ELOB antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Monoclonal antibodies provide a defined epitope recognition profile that can support consistent comparisons across experiments.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
