| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, food, agriculture, and environment |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of acetic acid or acetate and evaluation of drug effects on acetate metabolism. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Serum, plasma, food, agriculture, and environment. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, food, agriculture, and environment, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.13 mM for interpreting low-signal samples.
- Feature emphasis: Sensitive. Use as little as 10 µL samples. Detection range: 0.20 to 20 mM acetate for colorimetric assays and 0.13 to 2 mM for fluorimetric assays.
Additional feature notes highlight Simple. The assay involves adding a single working reagent, incubation for 30 min at room temperature, and read; Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of acetate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Acetate is a common anion and fundamental to all forms of life. When bound to coenzyme A, it is central to the metabolism of carbohydrates and fats. It is acid form, acetic acid, is produced and excreted by acetic acid bacteria, such as Acetobacter genus and Clostridium acetobutylicum, which are found universally in foodstuffs, water, and soil. Acetic acid is also a component of the vaginal lubrication of humans and other primates, where it appears to serve as a mild antibacterial agent. Acetic acid is the main component of vinegar and is extensively used in food, dyes, paints, glue, and synthetic fibers. BioAssay Systems assay uses enzyme-coupled reactions to form a colored, fluorescent product. The color absorbance at 570nm or fluorescence intensity at 530nm/585nm is directly proportional to the acetate concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.13 mM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify acetate in serum, plasma, food by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, food handling.
- Monitor time-course or pre/post changes in serum, plasma, food across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Intradialytic acid-base changes and organic anion production during high versus low bicarbonate hemodialysis
Park, S., et al (2020). Intradialytic acid-base changes and organic anion production during high versus low bicarbonate hemodialysis. American Journal of Physiology. Renal Physiology, 318(6), F1418-F1429. Assay: Acetate in human plasma, serum and dialysate.
Memory cd8+ t cells balance pro- and anti-inflammatory activity by reprogramming cellular acetate handling at sites of infection
Balmer, M. L.,et al (2020). Memory cd8+ t cells balance pro- and anti-inflammatory activity by reprogramming cellular acetate handling at sites of infection. Cell Metabolism, 32(3), 457-467.e5. Assay: Acetate in human peritoneal fluid.
Post-ruminal supplies of glucose and casein, but not acetate, stimulate milk protein synthesis in dairy cows through differential effects on mammary metabolism
Danes, M. a. C., et al (2020). Post-ruminal supplies of glucose and casein, but not acetate, stimulate milk protein synthesis in dairy cows through differential effects on mammary metabolism. Journal of Dairy Science, 103(7), 6218-6232. Assay: Acetate in cow plasma.
Extended mutualism between termites and gut microbes: nutritional symbionts contribute to nest hygiene
Inagaki, T., & Matsuura, K. (2018). Extended mutualism between termites and gut microbes: nutritional symbionts contribute to nest hygiene. The Science of Nature, 105(9-10), 52. Assay: Acetate in termite gut content.
The dynamics of the metabolism of acetate and bicarbonate associated with use of hemodialysates in the ABChD trial: a phase IV, prospective, single center, single blind, randomized, cross-over, two week investigation
Smith, W. B., Gibson, S., Newman, G. E., Hendon, K. S., Askelson, M., Zhao, J., & Thadhani, R. I. (2017). The dynamics of the metabolism of acetate and bicarbonate associated with use of hemodialysates in the ABChD trial: a phase IV, prospective, single center, single blind, randomized, cross-over, two week investigation. BMC Nephrology, 18(1), 273. Assay: Acetate in human blood.
Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling
Cuervo-Soto, LI et al (2015). Identification of a novel carbohydrate esterase from Bjerkandera adusta: Structural and function predictions through bioinformatics analysis and molecular modeling. Proteins: Structure, Function, and Bioinformatics 83(3): 533-546. Assay: Acetate in microbe culture.
Utilization of galactooligosaccharides by Bifidobacterium longum subsp
Garrido D et al (2013). Utilization of galactooligosaccharides by Bifidobacterium longum subsp. infantis isolates. Food Microbiol. 33(2):262-70. Assay: Acetate in microbe cell.
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