| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | biological samples (cells, tissues, etc) |
| Shipping | |
| Species | |
| Storage |
Overview
For sensitive quantitative determination of adipogenesis and high-throughput screening of adipogenesis modulators. The assay uses OD570nm or FL530/585nm for signal readout. Compatible sample input includes biological samples (cells, tissues, etc). Typical stated assay timing is 30min.
Key elements and design rationale
- Readout format: OD570nm or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes biological samples (cells, tissues, etc), which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.16 nmoles (colorimetric assay); 0.075 nmoles (fluorimetric assay) for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 40 µL samples. Linear detection range in 96-well plate: 0.16 to 5 nmoles for colorimetric assays and 0.075 to 0.5 nmoles for fluorimetric assays.
Additional feature notes highlight Fast and convenient. The addition of a single working reagent and 30-min incubation procedure combines sample extraction, hydrolysis and color reaction. The whole procedure is performed at room temperature with NO incubator or heating needed ; Robust and amenable to HTS. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 200 Tests.
Biological background
This product is centered on measurement of adipogenesis within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Adipogenesisis a tightly regulated cellular differentiation process, in which mesenchymal stem cells commit to preadipocytes and preadipocytes differentiate into adipocytes. Adipocytes, processing the largest energy reserve as triglycerol in the body of animals, play a key role in energy homeostasis. An increasingly sedentary lifestyle coupled with an energy-rich diet has contributed to a high frequency of obesity and other health problems, such as type 2 diabetes. Simple, direct and automation-ready procedures for measuring adipogenesis find wide applications in research and drug discovery. BioAssay Systems’ EAPG-200 Assay Kit determines adipogenesis, in which triglycerides are extracted, hydrolyzed to glycerol and measured using a Dye Reagent. The color intensity at 570nm or fluorescence intensity at FL530/585nm is directly proportional to glycerol concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.16 nmoles (colorimetric assay); 0.075 nmoles (fluorimetric assay).
Procedures and timing
Stated procedure or timing information: 30min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify adipogenesis in cells, tissues by OD570 nm or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched cells, tissues handling.
- Monitor time-course or pre/post changes in cells, tissues across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
