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| Sample Type(s) | Serum |
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Overview
For quantitative determination of HDL and LDL/VLDL cholesterol and evaluation of drug effects on HDL and LDL/VLDL metabolism. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Serum. Typical stated assay timing is 60 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of OD, FL: 1, 0.2 mg/dL for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection range in 96-well plate: 1 to 100 mg/dL cholesterol for colorimetric assays and 0.2 to 10 mg/dL for fluorimetric assays.
Additional feature notes highlight Convenient. Room temperature assay. No 37°C heater is needed. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of af hdl and ldl/vldl within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
CHOLESTEROL concentrations in High-Density Lipoprotein (HDL) and Low-Density (LDL)/Very-Low-Density (VLDL) Lipoproteins are strong predictors for coronary heart disease. Functional HDL offers protection by removing cholesterol from cells and atheroma. Higher concentrations of LDL and lower concentrations of functional HDL are strongly associated with cardiovascular disease due to higher risk of atherosclerosis. The balances between high- and low-density lipoproteins are solely genetically determined, but can be changed by medications, food choices and other factors. Simple, direct and automation-ready procedures for measuring HDL and LDL/VLDL concentrations are very desirable. BioAssay Systems HDL and LDL/VLDL quantification kit is based on our improved PEG precipitation method in which HDL and LDL/VLDL are separated, and cholesterol concentrations are determined using a single Working Reagent that combines cholesterol ester hydrolysis, oxidation and color reaction in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to total cholesterol concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit(s): Colorimetric: 1 mg/dL / Fluorescent: 0.2 mg/dL. Additional source wording: OD, FL: 1, 0.2 mg/dL.
Procedures and timing
Stated procedure or timing information: 60 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify af hdl and ldl/vldl in serum by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched serum handling.
- Monitor time-course or pre/post changes in serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
I want to ask about the differences between “EnzyChrom™ AF HDL and LDL/VLDL Assay Kit and EnzyChrom™ HDL and LDL/VLDL Assay Kit. Since I want to work on mice.
Both kits will work with mouse serum and plasma samples, but I would recommend using serum, because we have not validated the use of plasma. The EHDL-100 kit has a linear detection range of 5 -300 mg/dL. It requires a microplate reader with a 340nm filter. The E2HL-100 kit is more sensitive with a linear detection range of 1 -100 mg/dL using the colorimetric readout (requires a 570 nm filter), or 0.2 -10 mg/dL with the fluorimetric readout (requires a fluorescence microplate reader).
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis
Colijn, S et al. (2020). Cell-specific and athero-protective roles for RIPK3 in a murine model of atherosclerosis. Disease Models & Mechanisms, 13(1). Assay: HDL/LDL in mouse plasma.
Biocompatible modified water as a non-pharmaceutical approach to prevent metabolic syndrome features in obesogenic diet-fed mice
Lambert, K et al. (2020). Biocompatible modified water as a non-pharmaceutical approach to prevent metabolic syndrome features in obesogenic diet-fed mice. Food and Chemical Toxicology: An International Journal Published for the British Industrial Biological Research Association, 141, 111403. Assay: HDL/LDL in mouse serum and liver.
Low basal metabolic rate as a risk factor for development of insulin resistance and type 2 diabetes
Maciak, S et al. (2020). Low basal metabolic rate as a risk factor for development of insulin resistance and type 2 diabetes. BMJ Open Diabetes Research & Care, 8(1). Assay: HDL/LDL in mouse plasma.
IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity
Feng, J., Li, L., Ou, Z., Li, Q., Gong, B., Zhao, Z. & Yang, X. (2018). IL-25 stimulates M2 macrophage polarization and thereby promotes mitochondrial respiratory capacity and lipolysis in adipose tissues against obesity. Cellular & molecular immunology, 15(5), 493. Assay: HDL and LDL/VLDL in mice tissue/plasma.
Inhibition of Adhesion Molecule Expression in Early Progression of Atherosclerosis in Dyslipidemia Model on Sprague Dawley rats by Darapladib
Heriansyah, T., Lutfiana, N. C., Sulfia, Y. H., Sholichah, Z., Lestari, R. D., Sargowo, D., & Wihastuti, T. A. (2018). Inhibition of Adhesion Molecule Expression in Early Progression of Atherosclerosis in Dyslipidemia Model on Sprague Dawley rats by Darapladib. International Journal of Pharmacology, 14(3), 444-450. Assay: HDL and LDL/VLDL in sprague dewley rats serum.
Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis
Heriansyah, T., Adam, A. A., Wihastuti, T. A., & Rohman, M. S. (2017). Elaborate evaluation of serum and tissue oxidized LDL level with darapladib therapy: A feasible diagnostic marker for early atherogenesis. Asian Pacific Journal of Tropical Biomedicine, 7(2), 134-138. Assay: HDL and LDL/VLDL in sprague dewley rats serum.
Metabolic risk factors in mice divergently selected for BMR fed high fat and high carb diets
Sadowska, J., Gebczynski, A. K., & Konarzewski, M. (2017). Metabolic risk factors in mice divergently selected for BMR fed high fat and high carb diets. PloS one, 12(2), e0172892. Assay: HDL and LDL/VLDL in mice serum.
Fermented red ginseng potentiates improvement of metabolic dysfunction in metabolic syndrome rat models
Kho, M., Lee, Y., Park, J., Kim, H., Yoon, J., Ahn, Y. & Kang, D. (2016). Fermented red ginseng potentiates improvement of metabolic dysfunction in metabolic syndrome rat models. Nutrients, 8(6), 369. Assay: HDL and LDL/VLDL in sprague dewley rats plasma.
Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black
Bourdon JA et al (2012) Hepatic and pulmonary toxicogenomic profiles in mice intratracheally instilled with carbon black nanoparticles reveal pulmonary inflammation, acute phase response, and alterations in lipid homeostasis. Toxicol Sci 127(2):474-484. Assay: HDLandLDL/VLDL in mouse plasma.
Reconstituted High-density Lipoprotein Infusion Modulates Fatty Acid Metabolism in Patients with Type 2 Diabetes Mellitus
Drew, B.G. et al (2011). Reconstituted High-density Lipoprotein Infusion Modulates Fatty Acid Metabolism in Patients with Type 2 Diabetes Mellitus. J Lipid Res. 52(3):572-81. Assay: HDL and LDL/VLDL in human blood.
Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats – Effect of hypertriglyceridemia and gender
Klusonova P, et al (2011). Local metabolism of glucocorticoids in Prague hereditary hypertriglyceridemic rats – Effect of hypertriglyceridemia and gender. Steroids 76(12):1252-9. Assay: HDL and LDL/VLDL in mouse blood.
Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion
Nagajyothi F et at (2011). Trypanosoma cruzi utilizes the host low density lipoprotein receptor in invasion. PLoS Negl Trop Dis. 1;5(2):e953. Assay: HDL and LDL/VLDL in mouse serum.
Antihyperlipidemic Activity of the Methanolic extract from the Stems of Tinospora cordifolia on Sprague dawley rats
Parveen, T.D. et al (2011). Antihyperlipidemic Activity of the Methanolic extract from the Stems of Tinospora cordifolia on Sprague dawley rats. Der Pharmacia Sinica 2 (1): 104-109. Assay: HDL and LDL/VLDL in rat blood.
Antihyperlipidemic and Body Fat-Lowering Effects of Silk Proteins with Different Fibroin/Sericin Compositions in Mice Fed with High Fat Diet
Seo, C-W. et al (2011). Antihyperlipidemic and Body Fat-Lowering Effects of Silk Proteins with Different Fibroin/Sericin Compositions in Mice Fed with High Fat Diet. J. Agric. Food Chem. 59 (8): 4192-4197. Assay: HDL and LDL/VLDL in mouse blood.
AC13, a C-terminal fragment of apolipoprotein A-I, is a candidate biomarker for microscopic polyangiitis
Takakuwa Y, et al (2011). AC13, a C-terminal fragment of apolipoprotein A-I, is a candidate biomarker for microscopic polyangiitis. Arthritis Rheum. 63(11):3613-24. Assay: HDL and LDL/VLDL in human blood.
Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs
Uddin, M.J. et al (2011). Detection of quantitative trait loci affecting serum cholesterol, LDL, HDL, and triglyceride in pigs. BMC Genetics 2011, 12:62. Assay: HDL and LDL/VLDL in pig serum.
Genetic Deletion of Chemokine Receptor Ccr6 Decreases Atherogenesis in ApoE-Deficient Mice
Wan, W. et al (2011). Genetic Deletion of Chemokine Receptor Ccr6 Decreases Atherogenesis in ApoE-Deficient Mice. Circ Res.109(4):374-81. Assay: HDL and LDL/VLDL in mouse serum.
Increased oxidative stress and altered substrate metabolism in obese children
Oliver SR, et al (2010). Increased oxidative stress and altered substrate metabolism in obese children. Int J Pediatr Obes. 2010 Oct;5(5):436-44. Assay: HDL and LDL/VLDL in human plasma.
Moderate kidney disease inhibits atherosclerosis regression
Ponda, M.P. et al (2010). Moderate kidney disease inhibits atherosclerosis regression. Atherosclerosis 210 (1): 57-62. Assay: HDL and LDL/VLDL in mouse blood.
Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis
Shanmugasundaram R, Selvaraj RK (2010). Dietary lutein and fish oil interact to alter atherosclerotic lesions in a Japanese quail model of atherosclerosis. J Anim Physiol Anim Nutr (Berl). [Epub ahead of print]. Assay: HDLandLDL/VLDL in bird quail blood.
Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity
Tam J, et al (2010). Peripheral CB1 cannabinoid receptor blockade improves cardiometabolic risk in mouse models of obesity. J Clin Invest. 120(8):2953-66. Assay: HDL and LDL/VLDL in mouse serum.
Medium-chain triglycerides impair lipid metabolism and induce hepatic steatosis in very long-chain acyl-CoA dehydrogenase (VLCAD)-deficient mice
Tucci, S. et al (2010). Medium-chain triglycerides impair lipid metabolism and induce hepatic steatosis in very long-chain acyl-CoA dehydrogenase (VLCAD)-deficient mice. Mol. Gen. Met. 101(1): 40-47. Assay: HDL and LDL/VLDL in mouse blood.
Use of Inhibitors of Toll-like receptors in the prevention and treatment of hyperchloesterolenia and hyperlipidemia and dreases related thereto
Zhu, F.G. et al (2010). Use of Inhibitors of Toll-like receptors in the prevention and treatment of hyperchloesterolenia and hyperlipidemia and dreases related thereto. US2010/0098685A1. Assay: HDL and LDL/VLDL in mouse blood.
GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats
Lee, S.M. et al (2008).GCG-rich tea catechins are effective in lowering cholesterol and triglyceride concentrations in hyperlipidemic rats. Lipids 43(5): 419-429. Assay: HDL and LDL/VLDL in rat blood.
