| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | biological samples (serum, cell lysate, etc) |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of aldehyde dehydrogenase activity in biological samples (e.g serum, cell lysate, etc). The assay uses OD565nm for signal readout. Compatible sample input includes biological samples (serum, cell lysate, etc). Typical stated assay timing is 30min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes biological samples (serum, cell lysate, etc), which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.2 U/L for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Linear detection range 0.2 – 25 U/L ALDH in a 96-well plate assay.
Additional feature notes highlight Convenient. The assay involves the addition of a single working reagent and can be completed in under an hour; Robust and amenable to HTS. Homogeneous “mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of aldehyde dehydrogenase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
ALDEHYDE DEHYDROGENASES(ALDHs) are a superfamily of oxidoreductases that catalyze the conversion of aldehydes to carboxylic acids. ALDH is crucial in the metabolism of alcohol as alcohol dehydrogenase breaks down ethanol to acetaldehyde. Acetaldehyde, which is toxic to the body, is in turn broken down by ALDH to acetic acid. Imbalances of ALDHs have been linked to both alcoholism and alcohol sensitivity in people.BioAssay Systems’ EADH-100 Kit is based on the enzymatic conversion of acetaldehyde to acetic acid and NADH by ALDH. The formed NADH is converted into a colored product, the absorbance of which, measured at 565 nm, is proportional to the enzyme activity in the reaction.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.2 U/L.
Procedures and timing
Stated procedure or timing information: 30min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify aldehyde dehydrogenase in serum, cell lysate by OD565 nm readout.
- Compare treatment or phenotype groups using matched serum, cell lysate handling.
- Monitor time-course or pre/post changes in serum, cell lysate across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with aldehyde dehydrogenase enzyme?
No, the user needs to provide their own purified enzyme.
I noticed that precipitate formed when I mixed the Working Reagent and cell lysate sample. Is this normal?
Yes, this should not be a problem. Try centrifuging the sample and using the clear supernatant to run the assay (e.g. 5 minutes at 14000 rpm).
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
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