| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological |
| Shipping | |
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| Storage |
Overview
For quantitative determination of coenzyme A (CoA) and evaluation of drug effects on CoA metabolism. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Biological. Typical stated assay timing is 60 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 3 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive. Use 10 µL samples. Linear detection range: colorimetric assay 5 – 1000 µM, fluorimetric assay 3 – 100 µM CoA.
Additional feature notes highlight Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of coenzyme a within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Coenzyme A (CoA) is involved in many important biological activities including the synthesis and oxidation of fatty acids, pyruvate oxidation in the citric acid cycle and many others. One of CoA’s most crucial roles is the carrying and transferring of acyl groups. BioAssay Systems method provides a simple, two-step and high-throughput assay for measuring CoA. In this assay, the first step enzymatically converts CoA to acyl-CoA and the second step oxidizes the acyl-CoA producing an enoyl-CoA and H2O2. The resulting H2O2reacts with a specific dye to form a pink colored product. The optical density at 570nm or fluorescence intensity (530/585 nm) is directly proportional to the CoA concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 3 µM.
Procedures and timing
Stated procedure or timing information: 60 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify coenzyme a in biological by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched biological handling.
- Monitor time-course or pre/post changes in biological across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
2, 3-Dimethoxy-5-methyl-p-benzoquinone (Coenzyme Q0) Disrupts Carbohydrate Metabolism of HeLa Cells by Adduct Formation with Intracellular Free Sulfhydryl-Groups, and Induces ATP Depletion and Necrosis
Takahashi, Takayuki, Yukitoshi Mine, and Tadashi Okamoto (2018). 2, 3-Dimethoxy-5-methyl-p-benzoquinone (Coenzyme Q0) Disrupts Carbohydrate Metabolism of HeLa Cells by Adduct Formation with Intracellular Free Sulfhydryl-Groups, and Induces ATP Depletion and Necrosis. Biological and Pharmaceutical Bulletin 41.12: 1809-1817. Assay: Coenzyme A in human cells.
