| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, saliva, etc |
| Shipping | |
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Overview
For quantitative determination of creatine and evaluation of drug effects on creatine metabolism. The assay uses OD570nm, or FL530/590nm for signal readout. Compatible sample input includes Serum, plasma, urine, saliva, etc. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/590nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, saliva, etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 4 µM for interpreting low-signal samples.
- Feature emphasis: High sensitivity and wide linear range. Use 10 µL sample. Linear detection range 4 to 1000 µM (colorimetric) or 0.5 to 50 µM (fluorimetric).
Additional feature notes highlight Homogeneous and simple procedure. A simple “mix-and-measure” procedure allows reliable quantitation of creatine within 30 minutes. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of creatine within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
CREATINE is present in vertebrates and helps to supply energy to muscles. In humans and animals, approximately half of creatine originates from food (mainly from fresh meat). Creatine supplementation has been investigated as a possible therapeutic approach for the treatment of muscular, neuromuscular, neurological, and neurodegenerative diseases. Simple, direct, and automation-ready procedures for measuring creatine are popular in research and drug discovery. BioAssay Systems creatine assay is based on enzymatic reactions leading to the formation of a pink-colored product. The optical density at 570 nm or fluorescence intensity at λ ex/em = 530/585 nm is directly proportional to the creatine concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/590 nm).
Detection limit and analytical sensitivity
Reported detection limit: 4 µM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify creatine in serum, plasma, urine, saliva by OD570 nm, or FL530/590 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine, saliva handling.
- Monitor time-course or pre/post changes in serum, plasma, urine, saliva across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Can this kit be used to measure creatine concentrations in cells?
Yes. To determine intracellular creatine concentrations, harvest cells (0.1-2 million cells per assay) in a 1.5mL tube, centrifuge 1-2 min at 3,000 rpm on a table centrifuge. Remove culture medium, wash cells quickly with cold PBS. Immediately remove any PBS. Prepare Working Reagent. Add 120 µL WR directly to the cell pellet and 80 µL WR to standards in 96-wells. Vortex cell sample tube 1 min, incubate 30 min at room temp. Centrifuge 5 min at 14,000 rpm. Transfer 100 µL supernatant to 96-well. Read OD570nm on a plate reader.
Notes: 1. cell number. It is prudent to run several doses of cells, e.g. 0.1, 1, 2×106 cells to determine optimal cell number to be used in subsequent assays.
Working Reagent should contain a lysis reagent. BioAssay Systems will supply this reagent upon request.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Guanidinoacetic Acid Regulates Myogenic Differentiation and Muscle Growth Through miR-133a-3p and miR-1a-3p Co-mediated Akt/mTOR/S6K Signaling Pathway
Wang, Yujie, et al (2018). Guanidinoacetic Acid Regulates Myogenic Differentiation and Muscle Growth Through miR-133a-3p and miR-1a-3p Co-mediated Akt/mTOR/S6K Signaling Pathway. International journal of molecular sciences 19.9: 2837. Assay: Creatine in murine cells.
Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model
Chan, Yiumo Michael, et al (2017). Substantial deficiency of free sialic acid in muscles of patients with GNE myopathy and in a mouse model. PloS one 12.3: e0173261. Assay: Creatine in human and mouse tissues.
Inhibition of bone resorption with rankl binding peptides
Holz, Josefin-Beate, and Alex Hemeryck (2017). Inhibition of bone resorption with rankl binding peptides. U.S. Patent Application No. 15/295,229. Assay: Creatine in human urine.
Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis
Schoborg, JA et al (2014). Substrate replenishment and byproduct removal improve yeast cell-free protein synthesis. Biotechnology Journal 9(5): 630-640. Assay: Creatine in yeast cells.
Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics
Qi S et al (2012). Comparison of the metabolic profiling of hepatitis B virus-infected cirrhosis and alcoholic cirrhosis patients by using (1) H NMR-based metabonomics. Hepatol Res 42(7):677-85. Assay: Creatine in human serum.
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