| Field | Specification |
|---|---|
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, saliva samples etc |
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Overview
For quantitative determination of ethanol and evaluation of drug effects on alcohol metabolism. The assay uses OD565nm (Enzymatic) for signal readout. Compatible sample input includes Serum, plasma, urine, saliva samples etc. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD565nm (Enzymatic) supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, saliva samples etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.000008 for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Detection limit 0.0008 vol % (140 µM or 8 ppm), linearity up to 0.1% ethanol in 96-well plate assay.
Additional feature notes highlight Convenient. The procedure involves adding working reagent, incubating for 30 min and stopping reaction. No 37°C heater is needed; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of ethanol within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Alcoholic drinks are among the daily consumed beverages. Studies have shown heavy alcohol consumption may lead to various forms of liver diseases and to increased mortality rates. Quantitative determination of alcohol (ethanol, C 2 H 5 OH) has applications in basic research, drug discovery, clinic studies and in the alcoholic industry. Simple, direct and automation-ready procedures for measuring ethanol concentration are very desirable. BioAssay Systems EnzyChrom™ ethanol assay kit is based on alcohol dehydrogenase catalyzed oxidation of ethanol, in which the formed NADH is coupled to the formazan (MTT) chromogen. The intensity of the product color, measured at 565 nm, is proportionate to the ethanol concentration in the sampl
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.000008.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify ethanol in serum, plasma, urine, saliva samples by OD565 nm (Enzymatic) readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine, saliva samples handling.
- Monitor time-course or pre/post changes in serum, plasma, urine, saliva samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the assay react with methanol?
No, the assay does essentially not cross-react with methanol. Only at high concentrations (more than 100 mM methanol) will there be any significant nonspecific signal.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Programmed increases in LXRalpha induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity
Chang, R. C., et al (2019). Programmed increases in LXRalpha induced by paternal alcohol use enhance offspring metabolic adaptation to high-fat diet induced obesity. Molecular Metabolism, 30, 161-172. Assay: Ethanol in mouse plasma.
Ethanol-induced changes in brain of transgenic mice overexpressing dyrk1a
Fructuoso, M., et al (2020). Ethanol-induced changes in brain of transgenic mice overexpressing dyrk1a. Molecular Neurobiology, 57(7), 3195-3205. Assay: Ethanol in mouse plasma.
A standardized polyherbal preparation POL-6 diminishes alcohol withdrawal anxiety by regulating Gabra1, Gabra2, Gabra3, Gabra4, Gabra5 gene expression of GABAA receptor signaling pathway in rats
Sharma, L., et al (2021). A standardized polyherbal preparation POL-6 diminishes alcohol withdrawal anxiety by regulating Gabra1, Gabra2, Gabra3, Gabra4, Gabra5 gene expression of GABAA receptor signaling pathway in rats. BMC Complementary Medicine and Therapies, 21(1), 13. Assay: Ethanol in rat plasma.
Unraveling the molecular mechanisms involved in alcohol intake and withdrawal in adolescent mice exposed to alcohol during early life stages
Montagud-Romero, S., et al (2021). Unraveling the molecular mechanisms involved in alcohol intake and withdrawal in adolescent mice exposed to alcohol during early life stages. Progress in Neuro-Psychopharmacology & Biological Psychiatry, 104, 110025 Assay: Ethanol in mouse plasma.
5-Aza-2′-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats
Qiao, Xiaomeng, et al (2017). 5-Aza-2′-deoxycytidine in the medial prefrontal cortex regulates alcohol-related behavior and Ntf3-TrkC expression in rats. PloS one 12.6: e0179469. Assay: Ethanol in rat plasma.
Alcohol exposure during pregnancy causes non-compaction cardiomyopathy in offspring mice
Zhou, Huiming, et al (2017). Alcohol exposure during pregnancy causes non-compaction cardiomyopathy in offspring mice. Journal of Third Military Medical University, 39(17): 1696-1701. Assay: Ethanol in mice serum.
Increased ethanol production by genetic engineering of microorganisms to express hemoglobin
Sanny, Tony, and Benjamin C. Stark (2016). Increased ethanol production by genetic engineering of microorganisms to express hemoglobin. U.S. Patent No. 9,309,540. Assay: Ethanol in microorganisms cell cuture.
Alcohol-induced behavior change in zebrafish models
Echevarria DJ, et al. (2011). Alcohol-induced behavior change in zebrafish models. Rev Neurosci. 22(1):85-93. Assay: Ethanol in zebrafish blood.
ATF4 and the integrated stress response are induced by ethanol and cytochrome P450 2E1 in human hepatocytes
Magne L, et al. (2011). ATF4 and the integrated stress response are induced by ethanol and cytochrome P450 2E1 in human hepatocytes. J Hepatol. 54(4):729-37. Assay: Ethanol in human hepatocytes.
Does acute alcohol exposure modulate aggressive behaviors in the zebrafish (Danio rerio), or is the bark worse than the bite
Echevarria,DJ and Hammack,CH (2010). Does acute alcohol exposure modulate aggressive behaviors in the zebrafish (Danio rerio), or is the bark worse than the bite. ijcp 23:62-69. Assay: Ethanol in zebrafish blood.
A novel role for glyceraldehyde-3-phosphate dehydrogenase and monoamine oxidase B cascade in ethanol-induced cellular damage
Ou XM, et al. (2010). A novel role for glyceraldehyde-3-phosphate dehydrogenase and monoamine oxidase B cascade in ethanol-induced cellular damage. Biol Psychiatry 67(9):855-63. Assay: Ethanol in human neuroblastoma cell.
Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha
Suwannarangsee S, et al. (2010). Characterization of alcohol dehydrogenase 1 of the thermotolerant methylotrophic yeast Hansenula polymorpha. Appl Microbiol Biotechnol. 88(2):497-507. Assay: Ethanol in yeast cell.
Alcohol Dose-Dependently Enhances 3 alpha-Androstanediol Formation in Frontal Cortex of Male rats Concomitant with Aggression
Pariset, JJ. al (2009). Alcohol Dose-Dependently Enhances 3 alpha-Androstanediol Formation in Frontal Cortex of Male rats Concomitant with Aggression. Open Neuropsychopharmacology 2:1-10. Assay: Ethanol in rat blood.
Paracrine activation of hepatic CB1 receptors by stellate cell-derived endocannabinoids mediates alcoholic fatty liver
Jeong WI et al (2008). Paracrine activation of hepatic CB1 receptors by stellate cell-derived endocannabinoids mediates alcoholic fatty liver. Cell Metab. 7(3):227-35. Assay: Ethanol in mouse serum.
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