| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum) |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of fumarate and drug effects on its metabolism. The assay uses OD565nm for signal readout. Compatible sample input includes Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum). Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD565nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food, beverage and other biological samples (e.g. cell lysate, tissue homogenate, serum), which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 5 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 20 µL sample. Linear detection range 0.005 to 2 mM fumarate in a 96-well plate assay.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading the optical density after 30 minutes. Room temperature assay. No 37°C heater is needed; High-throughput. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of fumarate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
FUMARATE, or Fumaric Acid, is one of the key components in the TCA cycle and is used by cells to form ATP. Human skin when exposed to sunlight will naturally produce fumaric acid. Fumarate is used as an additive by the food and beverage industries. Fumaric acid esters are also used to treat psoriasis. Increased urinary fumarate may be due to impaired Krebs cycle function, a defect in the enzyme fumarase, or mitochondrial function. BioAssay Systems fumarate assay kit is based on fumarase-catalyzed hydration of fumarate to malate. The malate is then oxidized by malate dehydrogenase generating NADH which reduces a formazan (MTT) dye. The intensity of the product color, measured at 565 nm is proportional to the fumarate concentration in the sample.
Detection method
Colorimetric (OD 565 nm).
Detection limit and analytical sensitivity
Reported detection limit: 5 µM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify fumarate in food, beverage (cell lysate, tissue by OD565 nm readout.
- Compare treatment or phenotype groups using matched food, beverage (cell lysate, tissue handling.
- Monitor time-course or pre/post changes in food, beverage (cell lysate, tissue across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Murine remote ischemic preconditioning suppresses diabetic ketoacidosis by enhancing glycolysis and entry into tricarboxylic acid cycle in the liver
Kurabayashi, A et al. (2020). Murine remote ischemic preconditioning suppresses diabetic ketoacidosis by enhancing glycolysis and entry into tricarboxylic acid cycle in the liver. Life Sciences, 253, 117748. Assay: Fumarate in mouse liver.
