| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, plasma, urine, saliva, milk, culture medium, food, beverages, etc |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of glucose and evaluation of drug effects on glucose metabolism. The assay uses OD340nm for signal readout. Compatible sample input includes Serum, plasma, urine, saliva, milk, culture medium, food, beverages, etc. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD340nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, plasma, urine, saliva, milk, culture medium, food, beverages, etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.1 mM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 20 µL samples. Linear detection range in 96-well plate: 0.1 to 3 mM (1.8 mg/dL to 54 mg/dL) glucose for colorimetric assays.
Additional feature notes highlight Convenient. Room temperature assay. No 37°C heater is needed; Simple and high-throughput. The procedure involves the addition of a single working reagent and incubation for 20 min at room temperature. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of glucose within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Glucose (C6H12O6) is a key diagnostic parameter for many metabolic disorders. Increased glucose levels have been associated with diabetes mellitus, and hyperactivity of the thyroid, pituitary, and adrenal glands. Decreased levels are found in insulin-secreting tumors, myxedema, hypopituitarism, and hypoadrenalism. Simple, direct, and high-throughput assays for measuring glucose concentrations find wide applications in research and drug discovery. BioAssay Systems glucose assay kit II uses an enzyme to reduce NAD. The produced NADH, measured at 340 nm, is proportional to the glucose concentration in the sample
Detection method
Colorimetric (OD 340 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.1 mM.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify glucose in serum, plasma, urine by OD340 nm readout.
- Compare treatment or phenotype groups using matched serum, plasma, urine handling.
- Monitor time-course or pre/post changes in serum, plasma, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What are the differences between the three EnzyChrom Glucose assay kits?
EBGL – best at detecting low levels of glucose (as low as 5 µM)
EGL2 – best at measuring high levels of glucose using a low wavelength filter (340 nm)
EGL3 – best at measuring high levels of glucose using a high wavelength filter (565 nm)
Which of the three EnzyChrom Glucose assay kits can be used with urine, saliva, or serum samples?
EBGL – Not recommended for urine, saliva, or serum samples
EGL2 – Compatible with urine and serum samples, not recommended for saliva samples
EGL3 – Recommended for urine, saliva, and serum samples
*Sample blanks are to be used when testing urine samples with EGL2 or EGL3.
*Sample blanks are to be used when testing urine samples with EGL2 or EGL3
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
