| Field | Specification |
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| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological |
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Overview
For quantitative determination of glutathione peroxidase activity and evaluation of drug effects on GPX activity. The assay uses OD340nm for signal readout. Compatible sample input includes Biological. Typical stated assay timing is 20 min.
Key elements and design rationale
- Readout format: OD340nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 40 U/L for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use a 10 µL sample. Linear detection ranges from 40 to 800 U/L GPX activity.
Additional feature notes highlight Convenient and high-throughput. Homogeneous “mix-incubate-measure” type assay. Can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of glutahione peroxidase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Glutathione peroxidase (GPX, EC 1.11.1.9) represents an enzyme family with peroxidase activity whose main biological role is to protect the organism from oxidative damage. It helps prevent lipid peroxidation of cellular membranes by removing free peroxide in the cell. GPX catalyzes the following reaction with glutathione reductase (GR), Simple, direct, and high-throughput assays for GPX activity find wide applications. BioAssay Systems improved assay directly measures NADPH consumption in the enzyme-coupled reactions. The measured decrease in optical density at 340 nm is directly proportional to the enzyme activity in the sample.
Detection method
Colorimetric (OD 340 nm).
Detection limit and analytical sensitivity
Reported detection limit: 40 U/L.
Procedures and timing
Stated procedure or timing information: 20 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify glutahione peroxidase in biological by OD340 nm readout.
- Compare treatment or phenotype groups using matched biological handling.
- Monitor time-course or pre/post changes in biological across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Effects of abomasal infusion of essential fatty acids and conjugated linoleic acid on performance and fatty acid, antioxidative, and inflammatory status in dairy cows
Haubold, S et al. (2020). Effects of abomasal infusion of essential fatty acids and conjugated linoleic acid on performance and fatty acid, antioxidative, and inflammatory status in dairy cows. Journal of Dairy Science, 103(1): 972-991. Assay: Glutathione Peroxidase in bovine plasma.
Cecal metabolome fingerprint in a rat model of decompression sickness with neurological disorders
de Maistre, S et al. (2020). Cecal metabolome fingerprint in a rat model of decompression sickness with neurological disorders. Scientific Reports, 10(1), 15996. Assay: Glutathione Peroxidase in rat plasma.
Effects of feeding increasing levels of yerba mate on lamb meat quality and antioxidant activity
Pena-Bermudez, YA et al. (2020). Effects of feeding increasing levels of yerba mate on lamb meat quality and antioxidant activity. Animals: An Open Access Journal from MDPI, 10(9). Assay: Glutathione Peroxidase in lamb tissue.
Zinc regulates reactive oxygen species generation in platelets
Lopes-Pires, ME et al. (2020). Zinc regulates reactive oxygen species generation in platelets. Platelets: 1-10. Assay: Glutathione Peroxidase in human platelet suspensions.
Gallic acid attenuates isoniazid and rifampicin-induced liver injury by improving hepatic redox homeostasis through influence on Nrf2 and NF-kB signalling cascades in Wistar Rats
Sanjay, S et al. (2021). Gallic acid attenuates isoniazid and rifampicin-induced liver injury by improving hepatic redox homeostasis through influence on Nrf2 and NF-kB signalling cascades in Wistar Rats. Journal of Pharmacy and Pharmacology, 73(4): 473-486. Assay: Glutathione Peroxidase in rat liver.
Hypermetabolism of glutathione, glutamate and ornithine via redox imbalance in methylglyoxal-induced peritoneal injury rats
Hirahara, I et al. (2020). Hypermetabolism of glutathione, glutamate and ornithine via redox imbalance in methylglyoxal-induced peritoneal injury rats. Journal of Biochemistry, 167(2), 185-194. Assay: Glutathione Peroxidase in rat peritoneal effluent.
Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets
Vieira da Silva, I et al. (2021). Glutamine and cystine-enriched diets modulate aquaporins gene expression in the small intestine of piglets. PloS One, 16(1), e0245739. Assay: Glutathione Peroxidase in pig serum.
Dietary supplementation of postbiotics mitigates adverse impacts of heat stress on antioxidant enzyme activity, total antioxidant, lipid peroxidation, physiological stress indicators, lipid profile and meat quality in broilers
Humam, AM et al. (2020). Dietary supplementation of postbiotics mitigates adverse impacts of heat stress on antioxidant enzyme activity, total antioxidant, lipid peroxidation, physiological stress indicators, lipid profile and meat quality in broilers. Animals: An Open Access Journal from MDPI, 10(6). Assay: Glutathione Peroxidase in avian plasma.
Heat-induced endoplasmic reticulum stress in soleus and gastrocnemius muscles and differential response to UPR pathway in rats
Sharma, S et al. (2021). Heat-induced endoplasmic reticulum stress in soleus and gastrocnemius muscles and differential response to UPR pathway in rats. Cell Stress & Chaperones, 26(2): 323-339. Assay: Glutathione Peroxidase in rat muscle.
Consumption of nixtamal from a new variety of hybrid blue maize ameliorates liver oxidative stress and inflammation in a high-fat diet rat model
Magana-Cerino, JM et al. (2020). Consumption of nixtamal from a new variety of hybrid blue maize ameliorates liver oxidative stress and inflammation in a high-fat diet rat model. Journal of Functional Foods, 72, 104075. Assay: Glutathione Peroxidase in rat liver.
Supplementation of postbiotic RI11 improves antioxidant enzyme activity, upregulated gut barrier genes, and reduced cytokine, acute phase protein, and heat shock protein 70 gene expression levels in heat-stressed broilers
Humam, AM et al. (2021). Supplementation of postbiotic RI11 improves antioxidant enzyme activity, upregulated gut barrier genes, and reduced cytokine, acute phase protein, and heat shock protein 70 gene expression levels in heat-stressed broilers. Poultry Science, 100(3), 100908. Assay: Glutathione Peroxidase in avian plasma.
Anti-inflammatory effects of ribes diacanthum pall mediated via regulation of nrf2/ho-1 and nf-kb signaling pathways in lps-stimulated raw 264
Kim, NY et al. (2020). Anti-inflammatory effects of ribes diacanthum pall mediated via regulation of nrf2/ho-1 and nf-kb signaling pathways in lps-stimulated raw 264. 7 macrophages and a tpa-induced dermatitis animal model. Antioxidants (Basel, Switzerland), 9(7). Assay: Glutathione Peroxidase in mouse cells.
Lactobacillus spp
Hsieh, P-S et al. (2021). Lactobacillus spp. Reduces ethanol-induced liver oxidative stress and inflammation in a mouse model of alcoholic steatohepatitis. Experimental and Therapeutic Medicine, 21(3), 188. Assay: Glutathione Peroxidase in mouse liver.
Stimulating fermentation by the prolonged acceleration of gut transit protects against decompression sickness
De Maistre, S., Vallee, N., Gaillard, S., Duchamp, C., & Blatteau, J. E. (2018). Stimulating fermentation by the prolonged acceleration of gut transit protects against decompression sickness. Scientific reports, 8(1), 10128. Assay: Glutahione peroxidase in rat plasma.
Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese
Medina-Leendertz, S., Mora, M., Vielma, J. R., Bravo, Y., Atencio-Bracho, L., Leal-Yepez, A. & Bonilla, E. (2018). Melatonin decreases oxidative stress in Drosophila melanogaster exposed to manganese. Investigacion Clinica, 59(3), 230-241. Assay: Glutahione peroxidase in D. melanogaster tissues.
Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster
Bonilla, E., Medina-Leendertz, S., Mora, M., Vielma, J. R., Atencio-Bracho, L., Bravo, Y. & Arcaya, J. L. (2017). Minocycline Protection against Paraquat Toxicity in Drosophila melanogaster. Toxicology International, 24(1), 65-73. Assay: Glutahione peroxidase in rat tissues.
Dietary hydroxycinnamates prevent oxidative damages to liver, spleen, and bone marrow cells in irradiation-exposed mice
Kook, S. H., Cheon, S. R., Kim, J. H., Choi, K. C., Kim, M. K., & Lee, J. C. (2017). Dietary hydroxycinnamates prevent oxidative damages to liver, spleen, and bone marrow cells in irradiation-exposed mice. Food science and biotechnology, 26(1), 279-285. Assay: Glutahione peroxidase in mice tissues.
Fatty acids, lipid and protein oxidation, metmyoglobin reducing activity and sensory attributes of biceps femoris muscle in goats fed a canola and palm oil blend
Adeyemi, K. D., Ismail, M., Ebrahimi, M., Sabow, A. B., Shittu, R. M., Karim, R., & Sazili, A. Q. (2016). Fatty acids, lipid and protein oxidation, metmyoglobin reducing activity and sensory attributes of biceps femoris muscle in goats fed a canola and palm oil blend. South African Journal of Animal Science, 46(2), 139-151. Assay: Glutahione peroxidase in goat tissues.
Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend
Adeyemi, K. D., Sabow, A. B., Aghwan, Z. A., Ebrahimi, M., Samsudin, A. A., Alimon, A. R., & Sazili, A. Q. (2016). Serum fatty acids, biochemical indices and antioxidant status in goats fed canola oil and palm oil blend. Journal of animal science and technology, 58(1), 6. Assay: Glutahione peroxidase in goat serum.
Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats
Adeyemi, K. D., Shittu, R. M., Sabow, A. B., Abubakar, A. A., Karim, R., Karsani, S. A., & Sazili, A. Q. (2016). Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of animal science and technology, 58(1), 23. Assay: Glutahione peroxidase in boer tissues.
Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus
Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutahione peroxidase in fish plasma.
Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus
Abdel-Latif, HMR and Khalil, RH (2014). Evaluation of two Phytobiotics, Spirulina platensis and Origanum vulgare extract on Growth, Serum antioxidant activities and Resistance of Nile tilapia (Oreochromis niloticus) to pathogenic Vibrio alginolyticus. Intern. J. Fisheries and Aquatic Studies 1(5): 250-255. Assay: Glutathione Peroxidase in fish plasma.
Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats
Ahmed, MA et al (2014). Pomegranate extract protects against cerebral ischemia/reperfusion injury and preserves brain DNA integrity in rats. Life Sciences 110(2): 61-9. Assay: Glutahione peroxidase in rat brain tissue.
Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats
Chen, W et al (2014). Lycium barbarum polysaccharides prevent memory and neurogenesis impairments in scopolamine-treated rats. PLoS One 9(2):e88076. Assay: Glutahione peroxidase in rat hippocampus tissue.
Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis rat Model
Effendy, NM and Shuid, AN (2014). Time and Dose-Dependent Effects of Labisia pumila on Bone Oxidative Status of Postmenopausal Osteoporosis rat Model. Nutrients 6(8): 3288-302. Assay: Glutahione peroxidase in rat bone tissue.
Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol
Shabir, H et al (2014). Amelioration of lead- and cadmium-induced rat tracheal hypercontraction by linalool and eugenol. Toxicological & Environmental Chemistry 96(2): 307-317. Assay: Glutahione peroxidase in rat.
Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets
Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione peroxidase in deer blood.
Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets
Aghwan, ZA et al (2013). Blood Haematology, Serum Thyroid Hormones and Glutathione Peroxidase Status in Kacang Goats Fed Inorganic Iodine and Selenium Supplemented Diets. Asian-Australasian Journal of Animal Sciences (AJAS) 26.11: 1577-1582. Assay: Glutahione Peroxdiase in Deer Blood.
Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol
Calamita G et al (2012). Biophysical assessment of aquaporin-9 as principal facilitative pathway in mouse liver import of glucogenetic glycerol. Biol Cell 104(6):342-51. Assay: Glutahione peroxidase in human blood.
The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma
Labib, HM, et al (2010). The Role of Oxidative Stress Markers and Nitric Oxide Levels in the Pathogenesis of Glaucoma. Austr. J. Basic and Applied Sci 4(8): 3553-3558. Assay: Glutathione Peroxidase in human blood.
