| Field | Specification |
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| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological |
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Overview
For quantitative determination of glycogen and evaluation of drug effects on glycogen metabolism. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Biological. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of OD, FL: 2, 0.2 µg/mL for interpreting low-signal samples.
Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of glycogen within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
GLYCOGEN is a branched polysaccharide of glucose units linked by α-1,4 glycosidic bonds and α-1,6 glycosidic bonds. It is stored primarily in the liver and muscle and forms an energy reserve that can be quickly mobilized to meet a sudden need for glucose. The most common glycogen metabolism disorder is found in diabetes, in which, due to abnormal amounts of insulin, liver glycogen can be abnormally accumulated or depleted. Genetic glycogen storage diseases have been associated with various inborn errors of metabolism caused by deficiencies of enzymes necessary for glycogen synthesis or breakdown. Simple, direct, and automation-ready procedures for measuring glycogen concentrations find wide applications in research and drug discovery. BioAssay Systems glycogen assay uses a single Working Reagent that combines the enzymatic breakdown of glycogen and the detection of glucose in one step. The color intensity of the reaction product at 570nm or fluorescence intensity at λex/em = 530/585 nm is directly proportional to the glycogen concentration in the sample. This simple convenient assay is carried out at room temperature and takes only 30 min.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit(s): Colorimetric: 2 µg/mL / Fluorescent: 0.2 µg/mL. Additional source wording: OD, FL: 2, 0.2 µg/mL.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify glycogen in biological by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched biological handling.
- Monitor time-course or pre/post changes in biological across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How to prepare cell samples for the glycogen assay?
We recommend a modified version of the protocol used by Murat & Serfaty (Clin Chem. 12:1576-7).
– Homogenize 10×6 cells with 200 µL buffer (0.025 M citrate, pH 4.2, 2.5 g/L NaF) on ice.
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– Centrifuge sample at 14,000 x g for 5 min to remove debris, and use 10 µL of the clear supernatant in the assay.
– I would also recommend diluting the standard in this buffer rather than distilled water to increase the accuracy of the assay.
Tissue preparation: 10 to 30 mg/mL tissue in 0.025 M citrate, ph3.2, 2.5 g/L NaF.
I obtained a very high glycogen concentration after homogenizing mouse liver tissue in a sucrose/EDTA buffer. Indeed, the amount of glycogen was higher than the amount of liver tissue I added. A sample blank (without enzyme A) showed there is only a small amount of glucose in the sample. What could cause these results?
One of the enzymes in enzyme A, transglucosidase, hydrolyzes sucrose into glucose and fructose. You are actually measuring the amount of buffer rather than the amount of glycogen in your sample. We recommend using a sucrose free buffer, e.g. (0.025 M citrate, ph3.2, 5 g/L NaF).
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Liver ChREBP protects against fructose-induced glycogenic Hepatotoxicity by regulating L-type pyruvate kinase
Shi, J., et al (2020). Liver ChREBP protects against fructose-induced glycogenic Hepatotoxicity by regulating L-type pyruvate kinase. Diabetes, 69(4), 591-602. Assay: Glycogen in mouse liver tissue.
Paradoxical pro-inflammatory responses by human macrophages to an amoebae host-adapted legionella effector
Price, C., et al (2020). Paradoxical pro-inflammatory responses by human macrophages to an amoebae host-adapted legionella effector. Cell Host & Microbe, 27(4), 571-584.e7. Assay: Glycogen in human macrophage cellls.
CaMKIV limits metabolic damage through induction of hepatic autophagy by CREB in obese mice
Liu, J., et al. (2020). CaMKIV limits metabolic damage through induction of hepatic autophagy by CREB in obese mice. Journal of Endocrinology, 244(2), 353-367. Assay: Glycogen in mouse serum.
Isolated plin5-deficient cardiomyocytes store less lipid droplets than normal, but without increased sensitivity to hypoxia
Li, Y., et al (2021). Isolated plin5-deficient cardiomyocytes store less lipid droplets than normal, but without increased sensitivity to hypoxia. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids, 1866(4), 158873. Assay: Glycogen in mouse cardiomyocytes and heart tissue.
Biocompatible modified water as a non-pharmaceutical approach to prevent metabolic syndrome features in obesogenic diet-fed mice
Lambert, K., et al. (2020). Biocompatible modified water as a non-pharmaceutical approach to prevent metabolic syndrome features in obesogenic diet-fed mice. Food and Chemical Toxicology, 141, 111403 Assay: Glycogen in mouse serum or liver tissue.
Nuclear receptor REVERBalpha is a state-dependent regulator of liver energy metabolism
Hunter, A. L., et al (2020). Nuclear receptor REVERBalpha is a state-dependent regulator of liver energy metabolism. Proceedings of the National Academy of Sciences, 117(41), 25869-25879. Assay: Glycogen in mouse liver tissue.
Amylases in the human vagina
Nunn, K. L., et al (2020). Amylases in the human vagina. mSphere, 5(6). Assay: Glycogen in cervicovaginal mucus.
Transmembrane protein 135 (TMEM135) is a liver X receptor target gene that mediates an auxiliary peroxisome matrix protein import pathway
Renquist, B. J., Madanayake, T. W., Ghimire, S., Geisler, C. E., Xu, Y., & Bogan, R. L. (2018). Transmembrane protein 135 (TMEM135) is a liver X receptor target gene that mediates an auxiliary peroxisome matrix protein import pathway. bioRxiv, 334979. Assay: Glycogen in mouse liver tissue.
Effect of Preslaughter Shackling on Stress, Meat Qu ality Traits, and Glycolytic Potential in Broilers
Turkyilmaz, M. K., Dereli Fidan, E., Karaarslan, S., Unubol Aypak, S., & Nazligul, A. (2018). Effect of Preslaughter Shackling on Stress, Meat Qu ality Traits, and Glycolytic Potential in Broilers. Assay: Glycogen in broiler chicken muscle tissue.
Metabolic Flux Analysis of the Synechocystis sp
Nakajima, T., Yoshikawa, K., Toya, Y., Matsuda, F., & Shimizu, H. (2017). Metabolic Flux Analysis of the Synechocystis sp. PCC 6803 Delta nrtABCD Mutant Reveals a Mechanism for Metabolic Adaptation to Nitrogen-Limited Conditions. Plant and Cell Physiology, 58(3), 537-545. Assay: Glycogen in Synechocystis cells.
Effects of octreotide on hepatic glycogenesis in rats with high fat diet-induced obesity
Wang, X. X., Ye, T., Li, M., Li, X., Qiang, O., Tang, C. W., & Liu, R. (2017). Effects of octreotide on hepatic glycogenesis in rats with high fat diet-induced obesity. Molecular medicine reports, 16(1), 109-118. Assay: Glycogen in Sprague Dewley rats liver tissue.
Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats
Adeyemi, K. D., Shittu, R. M., Sabow, A. B., Abubakar, A. A., Karim, R., Karsani, S. A., & Sazili, A. Q. (2016). Comparison of myofibrillar protein degradation, antioxidant profile, fatty acids, metmyoglobin reducing activity, physicochemical properties and sensory attributes of gluteus medius and infraspinatus muscles in goats. Journal of animal science and technology, 58(1), 23. Assay: Glycogen in mice liver tissue.
Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of gluteus medius muscle in goats
Adeyemi, K. D., Shittu, R. M., Sabow, A. B., Ebrahimi, M., & Sazili, A. Q. (2016). Influence of diet and postmortem ageing on oxidative stability of lipids, myoglobin and myofibrillar proteins and quality attributes of gluteus medius muscle in goats. PloS one, 11(5), e0154603. Assay: Glycogen in goat muscle tissue.
New links between SOD1 and metabolic dysfunction from a yeast model of amyotrophic lateral sclerosis
Bastow, E. L., Peswani, A. R., Tarrant, D. S., Pentland, D. R., Chen, X., Morgan, A. & Tuite, M. F. (2016). New links between SOD1 and metabolic dysfunction from a yeast model of amyotrophic lateral sclerosis. J Cell Sci, 129(21), 4118-4129. Assay: Glycogen in yeast cells.
Insulin-inducible SMILE inhibits hepatic gluconeogenesis
Lee, J. M., Seo, W. Y., Han, H. S., Oh, K. J., Lee, Y. S., Kim, D. K. & Koo, S. H. (2016). Insulin-inducible SMILE inhibits hepatic gluconeogenesis. Diabetes, 65(1), 62-73. Assay: Glycogen in mice liver tissue.
Inappropriate expression of the translation elongation factor 1A disrupts genome stability and metabolism
Tarrant, D. J., Stirpe, M., Rowe, M., Howard, M. J., von der Haar, T., & Gourlay, C. W. (2016). Inappropriate expression of the translation elongation factor 1A disrupts genome stability and metabolism. J Cell Sci, 129(24), 4455-4465. Assay: Glycogen in yeast cells.
Genetic variants in glucocorticoid and mineralocorticoid receptors are associated with concentrations of plasma cortisol, muscle glycogen content, and meat quality traits in male Nellore cattle
Poleti, M.D., et al (2015). Genetic variants in glucocorticoid and mineralocorticoid receptors are associated with concentrations of plasma cortisol, muscle glycogen content, and meat quality traits in male Nellore cattle. Domest Anim Endocrinol 51:105-13. Assay: Glycogen in bovine muscle tissue.
Long-term dietary effects on substrate selection and muscle fiber type in very-long-chain acyl-CoA dehydrogenase deficient (VLCAD-/-) mice
Tucci, S., Pearson, S., Herebian, D., & Spiekerkoetter, U. (2013). Long-term dietary effects on substrate selection and muscle fiber type in very-long-chain acyl-CoA dehydrogenase deficient (VLCAD-/-) mice. Biochimica et Biophysica Acta (BBA)-Molecular Basis of Disease 1832(4): 509-516. Assay: Glycogen in mouse skeletal muscle, liver.
Glycogen is the primary source of glucose during the lag phase of E
Yamamotoya, T., Dose, H., Tian, Z., Faure, A., Toya, Y., Honma, M.,.& Matsuno, H. (2012). Glycogen is the primary source of glucose during the lag phase of E. coli proliferation. Biochimica et Biophysica Acta (BBA)-Proteins and Proteomics 1824(12): 1442-1448. Assay: Glycogen in human buccal, skin, vaginal eluate.
Atorvastatin treatment reduces exercise capacities in rats: involvement of mitochondrial impairments and oxidative stress
Bouitbir, J., Charles, A. L., Rasseneur, L., Dufour, S., Piquard, F., Geny, B., & Zoll, J. (2011). Atorvastatin treatment reduces exercise capacities in rats: involvement of mitochondrial impairments and oxidative stress. Journal of Applied Physiology 111(5): 1477-1483. Assay: Glycogen in rat muscle tissue.
Inducible astrocytic glucose transporter-3 contributes to the enhanced storage of intracellular glycogen during reperfusion after ischemia
Iwabuchi S, Kawahara K (2011). Inducible astrocytic glucose transporter-3 contributes to the enhanced storage of intracellular glycogen during reperfusion after ischemia. Neurochem Int 59(2):319-25. Assay: Glycogen in rat astrocytes.
“Hepatic and muscular effects of different dietary fat content in VLCAD deficient mice
Primassin, S., S. Tucci, et al. (2011). “Hepatic and muscular effects of different dietary fat content in VLCAD deficient mice.” Mol Genet Metab 104(4): 546-51. Assay: Glycogen in mouse muscle, liver.
Metformin improves cardiac function in a nondiabetic rat model of post-MI heart failure
Yin, M., et al. (2011). Metformin improves cardiac function in a nondiabetic rat model of post-MI heart failure. Am J Physiol Heart Circ Physiol 301(2):H459-68. Assay: Glycogen in rat cardiac muscle.
