| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological, environment (soil), etc |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of invertase/sucrase enzyme activity. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Biological, environment (soil), etc. Typical stated assay timing is 40 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological, environment (soil), etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.007 U/L for interpreting low-signal samples.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight Sensitive and accurate. As low as 0.007 U/L invertase activity can be quantified; Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of invertase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Invertase (b-fructofuranosidase, EC 3.2.1.26) is an enzyme that catalyzes the hydrolysis of sucrose to fructose and glucose. Invertases cleave at the O-C(fructose) bond, whereas a related enzyme sucrase (EC 3.2.1.48) cleaves at the O-C(glucose) bond. A wide range of microorganisms produce invertase and can, thus, utilize sucrose as a nutrient. Invertase assay finds wide applications in environmental (e.g. soil), agricultural, and food (confectionery) industries. BioAssay Systems Invertase Assay Kit provides a convenient and ultra-sensitive colorimetric and fluorimetric means to measure invertase activity. In the assay, invertase cleaves sucrose, resulting in the formation of fructose and glucose, which is determined by a colorimetric (570nm) or fluorimetric method (λex/em = 530/585nm). The assay is simple, sensitive, stable, and high-throughput adaptable.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.007 U/L.
Procedures and timing
Stated procedure or timing information: 40 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify invertase in biological, environment (soil) by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched biological, environment (soil) handling.
- Monitor time-course or pre/post changes in biological, environment (soil) across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Can the EnzyChrom™ Invertase Assay be used to determine sucrase activity?
Yes, sucrase is the same as invertase.
How do I prepare honey samples?
Due to the high sugar content in honey, a 10 kDa cut-off centrifugal filter is needed in this experiment to remove background sugars (e.g. VWR Cat No: 82031-348).
1.) Accurately weigh out 10 mg of honey and dissolve it in 500 µL of H2O (50 fold dilution).
2.) Add 500 µL of the dissolved honey into the centrifugal filter. Centrifuge at 12,000 × g at 4°C until most of the liquid (there should be less than 50 µL left) has passed through the membrane (~20 min). Dispose of the eluent.
3.) To wash the invertase, add 500 µL of H2O to the tube and centrifuge at 12,000 × g at 4°C. Dispose eluent. Repeat wash one more time. Allow most of the liquid (less than 50 µL should remain) to pass through the filter.
4.) Dilute the concentrated invertase in the filter membrane by adding 500 µL of 1X reaction buffer. The sample is ready to be assayed. Refer to the EIVT product datasheet for instructions.
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Note: Levels of invertase may vary in honey samples, therefore if the signal is to high, serially dilute the invertase sample to determine the appropriate dilution to work with and multiply the calculated activity by the dilution factor. If the signal is too low, adjust the dilution in step 4.
Note
Adjusted Calculation: Invertase Activity: ((Rsample – Rcontrol)/(slope × t)) × (5×10-4 L/10 mg) (U/mg) Where 5 ×10-4 L comes from the 500 µL of reaction buffer added to dilute the concentrated invertase in the membrane and 10 mg was the initial mass of honey.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Effect of thermal treatment on the biochemical composition of tropical honey samples
Chua, L. S., et al (2014). Effect of thermal treatment on the biochemical composition of tropical honey samples. International Food Research Journal 21(2): 773-778. Assay: Invertase in honey.
Effect of thermal treatment on the biochemical composition of tropical honey samples
Chua, L. S., et al.(2014). Effect of thermal treatment on the biochemical composition of tropical honey samples. International Food Research Journal 21(2): 773-778. Assay: Invertase/Sucrase in Honey.
Exoproteome analysis of Starmerella bombicola results in the discovery of an esterase required for lactonization of sophorolipids
Ciesielska, K et al (2014). Exoproteome analysis of Starmerella bombicola results in the discovery of an esterase required for lactonization of sophorolipids. Journal of Proteomics(98):159-174. Assay: Invertase in S. bombicola protein extract.
