| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | All kinases |
| Shipping | |
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| Storage |
Overview
Rapid homogenous assay for protein kinase activity and high-throughput screen for kinase inhibitors. The assay uses FL530/585nm for signal readout. Compatible sample input includes All kinases. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes All kinases, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.01 U/L for interpreting low-signal samples.
- Feature emphasis: Safe. Non-radioactive assay.
Additional feature notes highlight Sensitive and accurate. As low as 0.01 U/L kinase can be quantified; Homogeneous and convenient. “Mix-incubate-measure” type assay. The whole assay involves adding a single working reagent and incubation for 10 min at room temperature. Available format information for this listing includes 400 Tests.
Biological background
This product is centered on measurement of kinase within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Kinases, also known as phosphotransferases, constitute a large family of enzymes that transfer phosphate groups from the high-energy donor molecule ATP to their specific substrates. Kinases are known to regulate the majority of cellular processes. The largest group of this family is the protein kinases. So far, 518 different kinases have been identified in humans, and up to 30% of human proteins are modified by these kinases. The enormous diversity and their key role in cellular signaling make them ideal targets for drug development. BioAssay Systems’ EnzyChrom™ Kinase Assay Kit provides a simple and rapid method for assaying kinase activity and high-throughput screening for kinase inhibitors. This homogeneous microplate-based assay involves incubating the kinase with a single working reagent, in which ADP is enzymatically converted to ATP and pyruvate, which is quantified using a fluorimetric (530nm/590nm) assay method.
Detection method
Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.01 U/L.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify kinase in all kinases by FL530/585 nm readout.
- Compare treatment or phenotype groups using matched all kinases handling.
- Monitor time-course or pre/post changes in all kinases across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Different p53 genotypes regulating different phosphorylation sites and subcellular location of CDC25C associated with the formation of polyploid giant cancer cells
Liu, K et al. (2020). Different p53 genotypes regulating different phosphorylation sites and subcellular location of CDC25C associated with the formation of polyploid giant cancer cells. Journal of Experimental & Clinical Cancer Research: CR, 39(1), 83. Assay: Kinase in cell cycle-related kinases.
Regulation of the rhaEWRBMA operon involved in l-rhamnose catabolism through two transcriptional factors, RhaR and CcpA, in Bacillus subtilis
Hirooka, K., Kodoi, Y., Satomura, T., & Fujita, Y. (2016). Regulation of the rhaEWRBMA operon involved in l-rhamnose catabolism through two transcriptional factors, RhaR and CcpA, in Bacillus subtilis. Journal of bacteriology, 198(5), 830-845. Assay: Kinase in B. subtilis (bateria) protein.
Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E
Zhang, X., Zhang, S., Wang, J., Zi, J., Wang, J., Chen, S., & Wan, Y. (2016). Expression, Purification, and Characterization of a Sucrose Nonfermenting 1-Related Protein Kinases 2 of Arabidopsis thaliana in E. coli-Based Cell-Free System. BioMed research international 2016:9469356. Assay: Kinase in E. coli snrk2.1 protein.
Analogs of Cinnamic Acid Benzyl Amide As Nonclassical Inhibitors of Activated JAK2 Kinase
Marcin, M et al (2014). Analogs of Cinnamic Acid Benzyl Amide As Nonclassical Inhibitors of Activated JAK2 Kinase. Curr Cancer Drug Targets. 14(7):638-51. Assay: Kinase in CABA analogs cells.
Analogs of Cinnamic Acid Benzyl Amide As Nonclassical Inhibitors of Activated JAK2 Kinase
Mielecki, M et al (2014). Analogs of Cinnamic Acid Benzyl Amide As Nonclassical Inhibitors of Activated JAK2 Kinase. Curr Cancer Drug Targets. 14(7):638-51. Assay: Kinase in human enzymes.
Soluble Expression and Purification of the Catalytic Domain of human Vascular Endothelial Growth Factor Receptor 2 in Escherichia coli
Wei, J et al (2014). Soluble Expression and Purification of the Catalytic Domain of human Vascular Endothelial Growth Factor Receptor 2 in Escherichia coli. Journal of Microbiology and Biotechnology 25(8): 1227-33. Assay: Kinase in E. coli cells.
Soluble Expression and Purification of the Catalytic Domain of human Vascular Endothelial Growth Factor Receptor 2 in Escherichia coli
Wei, J et al (2014). Soluble Expression and Purification of the Catalytic Domain of human Vascular Endothelial Growth Factor Receptor 2 in Escherichia coli. Journal of Microbiology and Biotechnology 25(8): 1227-33. Assay: VEGFR-2-CD tyrosine kinase expressed in E. coli.
