| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Food, beverage, and biological samples (e.g. serum, cell lysate, etc) |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of L-arginine in food, beverage, biological samples (e.g. serum, cell lysate, etc). Simple. Using our internal standard method and robust reagents, no sophisticated sample pretreatment is required. Sensitive. The assay uses OD450nm for signal readout. Compatible sample input includes Food, beverage, and biological samples (e.g. serum, cell lysate, etc). Typical stated assay timing is 90 min.
Key elements and design rationale
- Readout format: OD450nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Food, beverage, and biological samples (e.g. serum, cell lysate, etc), which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.066 mM for interpreting low-signal samples.
- Feature emphasis: Simple. Using our internal standard method and robust reagents, no sophisticated sample pretreatment is required.
Additional feature notes highlight Sensitive and accurate. Use as little as 10 μL samples. Linear detection range in 96-well plate: 0.066 to 3 mM for colorimetric assays; High-throughput. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of l-arginine within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
L-ARGININEis an amino acid essential for protein metabolism and primarily found in the active sites of various proteins. During nitrogen metabolism, arginase breaks down arginine into urea and ornithine. L-Arginine is also present in many protein-rich foods and beverages. In particular, its detection in beverages, such as juices and wine, is crucial for quality control.BioAssay Systems’ L-Arginine assay measures the urea generated from arginase hydrolysis of arginine with our proprietary reagents. The increase in absorbance at 450 nm is proportional to the arginine concentration.
Detection method
Colorimetric (OD 450 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.066 mM.
Procedures and timing
Stated procedure or timing information: 90 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify l-arginine in food, beverage, and biological samples (serum by OD450 nm readout.
- Compare treatment or phenotype groups using matched food, beverage, and biological samples (serum handling.
- Monitor time-course or pre/post changes in food, beverage, and biological samples (serum across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
What is the normal concentration of arginine in blood?
A normal range of arginine in the blood is 21-150 μM. For patients with argininemia, the arginine concentration is typically 3-4 times higher than normal.
My samples became turbid after adding the urea reagent. How can I fix it?
For some samples, addition of urea reagent may cause turbidity. Test several dilutions to determine the optimal sample dilution factor at which turbidity does not occur. If it still occurs, transfer sample to an Eppendorf tube and centrifuge for 5 minutes at 14,000 rpm. Transfer supernatant back to reaction plate and read the absorbance.
What factors could potentially interfere with this assay?
Low pH of the sample could interfere with this assay since the pH optimum of arginase is between pH 9.0 and 10.0. Beverage samples with low pH (e.g. fruit juices) must be neutralized with 6N NaOH before running the assay.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
