| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Serum, urine, food, and beverages, etc |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of maltose and evaluation of drug effects on its metabolism. The assay uses OD570nm, or FL530/585nm for signal readout. Compatible sample input includes Serum, urine, food, and beverages, etc. Typical stated assay timing is 60 min.
Key elements and design rationale
- Readout format: OD570nm, or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Serum, urine, food, and beverages, etc, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 2 µM for interpreting low-signal samples.
- Feature emphasis: Fast and sensitive. Use of 10 µL sample. Linear detection range from 2 to 500 µM maltose for colorimetric assays and 1 to 50 µM for fluorimetric assays.
Additional feature notes highlight Convenient. The procedure involves adding a single working reagent and reading the absorbance after 60 minutes. Room temperature assay. No 37°C heater is needed; High-throughput. “Add-mix-read” type assay. Can be readily automated as a high-throughput 96-well plate assay for thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of maltose within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
MALTOSE(C12H22O11) is a disaccharide, composed of two glucose units linked by an α bond. It is produced from the hydrolysis of glycogen or starch, serving as a source of energy for plants and animals. Maltose can be found in foods such as grains, and other processed products. BioAssay Systems maltose assay provides a simple, one-step assay for measuring maltose. In this assay, maltose is converted to two glucose, which are then oxidized to form a colored product. The color intensity of the product at 570 nm or fluorescence at λex/em = 530/585 nm is directly proportional to maltose concentration in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 2 µM.
Procedures and timing
Stated procedure or timing information: 60 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify maltose in serum, urine, food, and beverages by OD570 nm, or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched serum, urine, food, and beverages handling.
- Monitor time-course or pre/post changes in serum, urine, food, and beverages across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
