| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Compounds that affect monoamine oxidase (MAO) activity |
| Shipping | |
| Species | |
| Storage |
Overview
For evaluation and high-throughput screen (HTS) of monoamine oxidase modulators. The assay uses FL530/585nm for signal readout. Compatible sample input includes Compounds that affect monoamine oxidase (MAO) activity. Typical stated assay timing is 35 min.
Key elements and design rationale
- Readout format: FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Compounds that affect monoamine oxidase (MAO) activity, which is useful when aligning matrix type with calibration and control design.
- Workflow timing: The listed assay time of 35 min helps frame batch planning, replicate handling, and plate throughput.
- Feature emphasis: Homogeneous and convenient. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
Additional feature notes highlight Robust and amenable to HTS: can be readily automated on HTS liquid handling systems for processing thousands of samples per day. Available format information for this listing includes 100 Tests (Custom bulk sizes available upon request).
Biological background
This product is centered on measurement of monoamine oxidase inhibitor screening within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
MONOAMINE OXIDASES(MAO, EC 1.4.3.4) are a family of mitochondrial enzymes that catalyze the oxidative deamination of monoamines. Two isoforms of MAO exist, MAO-A and MAO-B, with different inhibitor selectivity and tissue distribution. MAO dysfunction is thought to be responsible for a number of neurological disorders. Unusually high or low levels of MAOs in the body have been associated with depression, schizophrenia, substance abuse, attention deficit disorder, migraines, and irregular sexual maturation. MAO inhibitors are one of the major classes of drugs prescribed for the treatment of depression, Parkinson’s, and Alzheimer’s diseases. BioAssay Systems MAO Inhibitor Screening Assay Kit provides a convenient fluorimetric means to screen for MAO enzyme inhibitors. In the assay, MAO reacts withp-tyramine, a substrate for both MAO-A and MAO-B, resulting in the formation of H2O2, which is determined by a fluorimetric method (λem/ex= 585/530 nm). The assay is simple, sensitive, stable, and high-throughput adaptable.
Detection method
Fluorescent (FL 530/585 nm).
Procedures and timing
Stated procedure or timing information: 35 min.
Screening services
BioAssay Systems offers comprehensive Monoamine Oxidase lead discovery services, including compound library screening, inhibitor profiling, and high-throughput assays to identify potential drug candidates. We provide detailed analyses of compound activity, potency, and selectivity, supporting hit-to-lead and lead optimization efforts. Please contact us by Service .
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify monoamine oxidase inhibitor screening in compounds that affect monoamine oxidase (MAO) by FL530/585 nm readout.
- Compare treatment or phenotype groups using matched compounds that affect monoamine oxidase (MAO) handling.
- Monitor time-course or pre/post changes in compounds that affect monoamine oxidase (MAO) across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does the kit come with MAO-A and MAO-B enzymes or control MAO inhibitors?
The kit does not comes with either MAO enzyme. However, it comes with a clorgyline and pargylineas control inhibitors for MAO A and MAO B respectively.
How do I store the kit?
Store kit at -20°C for long term storage.
What species of MAO was the kit optimized for?
The kit was optimized for human MAO.
What if I am interested in testing inhibition of a species besides human?
We recommend that you experimentally determine the Km and then adjust the volume of substrate in the Working Reagent so that the final concentration of the substrate in the 100µL Reaction Volume is near the Km.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
