| Field | Specification |
|---|---|
| Mfr No | |
| Assay Time | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | urine, animal, and plant tissue samples |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of oxalate (oxalic acid) in urine and other samples. The assay uses OD595nm for signal readout. Compatible sample input includes urine, animal, and plant tissue samples. Typical stated assay timing is 10 min.
Key elements and design rationale
- Readout format: OD595nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes urine, animal, and plant tissue samples, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 20 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate for 10-minute incubation: 20 to 1500 µM oxalate.
Additional feature notes highlight Fast and convenient. Sample pre-treatment is faster and easier than using activated carbon in competitor assay kits; High-throughput adaptable. The procedure involves the addition of a single working reagent and incubation for 10 min at room temperature. Can be automated for processing thousands of samples per day. Available format information for this listing includes 100 Tests.
Biological background
This product is centered on measurement of oxalate within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Oxalate or Oxalic Acid is a metabolic breakdown product of the Kreb’s Cycle in eukaryotes, and the glyoxylate cycle in other microorganisms. It can be found in the urine of humans and other mammals. Oxalate concentration can be used as a measure of kidney function where a high level of oxalate is an indicator of kidney stones which are primarily made of the insoluble salt calcium oxalate. Measuring oxalate is more accurate than measuring calcium as a marker for kidney stones because calcium is excreted at high concentrations even in normal urine. Simple and high-throughput assays for measuring oxalate concentration find wide applications. BioAssay Systems’ oxalate assay kit uses a single Working Reagent that combines the oxalate oxidase reaction and color reaction in one step. The change in color intensity of the reaction product at 595 nm is directly proportional to oxalate in the sample.
Detection method
Colorimetric (OD 595 nm).
Detection limit and analytical sensitivity
Reported detection limit: 20 µM.
Procedures and timing
Stated procedure or timing information: 10 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- The description supports intervention-focused study designs in which researchers compare baseline and perturbed conditions.
Common research applications
- Quantify oxalate in urine, animal, and plant tissue samples by OD595 nm readout.
- Compare treatment or phenotype groups using matched urine, animal, and plant tissue samples handling.
- Monitor time-course or pre/post changes in urine, animal, and plant tissue samples across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How do I store the kit?
This kit is shipped on ice. Upon receiving, please keep the kit in the freezer (-20 to -10°C).
What samples have you tested?
The assay kit has been tested with human serum, rat serum, human plasma, plant tissue samples, and human urine. Please follow the directions on the protocol for sample pretreatment.
How do I prepare cell or tissue samples for assays?
Follow the general protocol below for cell and tissue samples.
Cell Samples. Suspend about two million (2 × 106) harvested cells in 400 µL PBS on ice. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in a ice-water bath). The degree of cell lysis can be checked under a microscope. Centrifuge homogenate at 14,000 g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the linear range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
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Tissue Samples. Cut the tissue into small pieces and weigh 20-100 mg tissue, then add 200- 1000 µL ice-cold PBS. Lysis can be achieved by homogenization (10-20 passes in a Dounce homogenizer on ice) or by sonication (preferably performed in an ice water bath). The degree of tissue lysis can be checked under a microscope. Centrifuge homogenate at 14,000g for 10 min. Transfer the clear supernatant into a clean tube. It is prudent to run a pilot test of the sample at different dilutions. Choose a dilution with the readings in the detection range of the standard curve for further assays. Most samples can be stored at -80°C if not assayed immediately.
What is the benefit of using an internal standard?
Some assays encounter interference and the values cannot be compared to a standard curve. The use of an internal standard corrects for potential interference.
I don’t have the correct wavelength filter, what other wavelength(s) would work?
The assay can be measured at 550 – 600 nm with 595 nm as the peak. Reading outside this range will result in a significant decrease in sensitivity.
Can I store unused reagents for future use?
Yes, unused reagents can be stored according to the assay protocol. Repeated freeze/thaw cycles of reagents should be avoided. Working Reagents should be made fresh for each assay and used within 2 hours.
Do I need to use a standard or standard curve with each assay run?
Yes, it is highly recommended.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
Vine tendrils use contact chemoreception to avoid conspecific leaves
Fukano, Y. (2017). Vine tendrils use contact chemoreception to avoid conspecific leaves. Proceedings of the Royal Society B: Biological Sciences, 284(1850), 20162650. Assay: Oxalate in leaf tissue.
