| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts. |
| Shipping | |
| Species | |
| Storage |
Overview
Uric acid determination in biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts. Sensitive. Use 10 µL samples. Linear detection range: Colorimetric assay 15 - 1000 µM Uric Acid. Fluorimetric. The assay uses OD570 or FL530/585nm for signal readout. Compatible sample input includes Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts.. Typical stated assay timing is 30 min.
Key elements and design rationale
- Readout format: OD570 or FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological samples such as cell culture media, biofluids (serum, urine), cell and tissue extracts., which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 4 μM for interpreting low-signal samples.
- Feature emphasis: Sensitive. Use 10 μL samples. Linear detection range: Colorimetric assay 15 – 1000 μM Uric Acid. Fluorimetric assay 4 – 300 μM Uric Acid.
Additional feature notes highlight Fast. Run time is 30 minutes for Optical Density method and 10 minutes for Fluorimetric method for rapid results; Convenient. Room temperature “mix-and-read” procedure can be readily automated for high-throughput assay of thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of uric acid within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
URIC ACIDis the breakdown product of purines such as adenosine and inosine. Humans and other primates are incapable of further metabolizing uric acid. In humans, uric acid is excreted via the kidneys, but a failure to remove excess uric acid can lead to conditions such as gout or uric acid kidney stones.BioAssay Systems’ method provides a simple and high-throughput assay for measuring uric acid levels. In this assay, uric acid is enzymatically converted to allantoin, releasing H2O2. The resulting H2O2reacts with a specific dye to form a pink-colored product. The change in OD570nm or fluorescence intensity at λex/em = 530/585nm is directly proportional to the uric acid present in the sample.
Detection method
Colorimetric (OD 570 nm) or Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 4 μM.
Procedures and timing
Stated procedure or timing information: 30 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify uric acid in serum, urine by OD570 or FL530/585 nm readout.
- Compare treatment or phenotype groups using matched serum, urine handling.
- Monitor time-course or pre/post changes in serum, urine across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Can linear curve fitting be used to quantify uric acid?
Yes, at higher concentrations linear fitting can work, but in most cases, hyperbolic curve fitting provides the most accurate results due to the enzymatic nature of this assay.
Can I use Microsoft Excel or Google Sheets to do the hyperbolic curve fitting to determine the uric acid quantification?
Unfortunately, no. Neither Microsoft Excel nor Google Sheets has the ability to do hyperbolic curve fitting. To obtain a hyperbolic equation (y = (m × x)/(k + x)), the standard curve values must be exported to an application such as GraphPad or an online curve fitting tool such as mycurvefit.com. Once you have the curve equation, you can employ What-If Analysis and the Goal Seek function to solve for your unknown concentrations.
Is there a way to increase the sensitivity of the assay?
Yes, the sample volume could be increased to anywhere from 20-40 μL. The volumes of the standards will need to be increased by the same volume. In all cases, the volume of the Working Reagent needs to be greater than the volume of the sample.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
