| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Biological Samples, Purified Enzyme |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of farnesyltransferase enzyme activity in biological samples. The assay uses FL340/550 nm for signal readout. Compatible sample input includes Biological Samples, Purified Enzyme. Typical stated assay timing is 60 min.
Key elements and design rationale
- Readout format: FL340/550 nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Biological Samples, Purified Enzyme, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.024 U/L for interpreting low-signal samples.
- Feature emphasis: Safe and convenient. Non-radioactive assay. “Mix-incubate-measure” type assay. No wash and reagent transfer steps are involved.
Additional feature notes highlight Sensitive and accurate. Linear detection range 0.024 – 3.2 U/L FTase in a 384-well plate assay; High-throughput. Can be readily automated to assay thousands of samples per day. Available format information for this listing includes 400 Tests in 384-well plate.
Biological background
This product is centered on measurement of enzyfluo farnesyltransferase activity within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
FARNESYLTRANSFERASE(FTase, EC 2.5.1.58) catalyzes the transfer of a farnesyl group from farnesyl pyrophosphate to the cysteine residue of the C-terminus of target proteins. When not properly regulated, farnesylated proteins, including the Ras superfamily of small GTPases, can lead to developmental disorders and cancer. Simple, direct and high-throughput activity assays find wide applications for cancer research, while BioAssay Systems’ EFTS-400 assay kit provides a convenient fluorimetric method for assaying FTase activity. In this assay, FTase reacts with farnesyl pyrophosphate and the dansyl-peptide substrate releasing a product that is measurable by fluorescence (λem/ex = 340/550 nm).
Detection method
Fluorescent (FL 340/550 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.024 U/L.
Procedures and timing
Stated procedure or timing information: 60 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify enzyfluo farnesyltransferase activity in biological Samples, Purified Enzyme by FL340/550 nm readout.
- Compare treatment or phenotype groups using matched biological Samples, Purified Enzyme handling.
- Monitor time-course or pre/post changes in biological Samples, Purified Enzyme across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
Does your EFTS-100 kit include a standard or calibrator?
We do not include a standard or calibrator. 4.3 is however provided as a conversion factor for determining maximum sample fluorescence. From there, activity can be calculated.
I only have a spectrophotometer that reads OD. Would it be possible to run this assay colorimetrically?
One of the main substrates in this assay is a dansyl peptide fluorophore so the assay must be run fluorometrically
Does the kit include FTase enzyme?
This kit does not include enzyme. The purpose of this assay is to determine the FTase activity of the sample. If a pure enzyme is desired, the customer can purchase it from e.g Jena Biosciences, cat# PR-102.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.