| Field | Specification |
|---|---|
| Mfr No | |
| Detection Method | |
| Product Type | |
| Sample Type(s) | Tissue and cell lysates |
| Shipping | |
| Species | |
| Storage |
Overview
For quantitative determination of fatty acyl-CoAs in tissue and cell lysates. Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.3 to 100 µM fatty acyl-CoA. The internal standard method. The assay uses FL530/585nm for signal readout. Compatible sample input includes Tissue and cell lysates. Typical stated assay timing is 40 min.
Key elements and design rationale
- Readout format: FL530/585nm supports plate-based signal acquisition and consistent comparison across matched samples.
- Sample compatibility: The stated sample scope includes Tissue and cell lysates, which is useful when aligning matrix type with calibration and control design.
- Analytical range context: The supplied specifications include a stated detection limit of 0.3 µM for interpreting low-signal samples.
- Feature emphasis: Sensitive and accurate. Use as little as 10 µL samples. Linear detection range in 96-well plate: 0.3 to 100 µM fatty acyl-CoA. The internal standard method minimizes potential sample matrix interferences.
Additional feature notes highlight Fast and convenient. Room temperature assay with a 40 min incubation. No 37°C incubator is needed; High-throughput. Homogeneous “mix-incubate-measure” type assay. It can be readily automated to assay thousands of samples per day. Available format information for this listing includes 100 Tests in 96-well plate.
Biological background
This product is centered on measurement of enzyfluo fatty acyl-coa within the matrices described for the assay. In practice, datasets from this type of format are typically interpreted by comparing relative signal, activity, or abundance across matched control and experimental groups rather than relying on a single value in isolation. Careful alignment of sample matrix, incubation window, and calibration strategy is important when comparing results across plates, operators, or study days.
More details
Fatty Acyl-CoAsare a combination of Coenzyme A covalently bound to long-chain fatty acids. Fatty acyl-CoAs serve as both a precursor for triglyceride and phospholipids synthesis and a product of lipid catabolism. Catabolically, they facilitate the transport of fatty acids to the mitochondria for shuttling via the carnitine shuttle and subsequent β-oxidation of the fatty acid. Conversion of fatty acids to fatty acyl-CoAs is necessary for the generation of ATP during periods of high energy demand. Elevated fatty acyl-CoA levels may indicate dysregulation of lipid metabolism such as due to insulin resistance. Certain genetic disorders may also affect fatty acyl-CoA levels.BioAssay Systems’ fluorimetric fatty acyl-CoA assay works using a combination of enzymes that utilize fatty acyl-CoA as a substrate, which is coupled to form a fluorescent product. The resulting fluorescence intensity at λexc/em= 530/585 nm is linear to the fatty acyl-CoA concentration in the sample.
Detection method
Fluorescent (FL 530/585 nm).
Detection limit and analytical sensitivity
Reported detection limit: 0.3 µM.
Procedures and timing
Stated procedure or timing information: 40 min.
Research relevance and current trends
- Plate-based quantification and side-by-side group comparison remain central use cases for this assay format.
- The product notes emphasize multi-sample throughput, making it relevant for screening-oriented and larger batch comparison studies.
- Short assay timing and plate compatibility support time-course or repeated-measure collection plans when handling is kept consistent.
Common research applications
- Quantify enzyfluo fatty acyl-coa in tissue and cell lysates by FL530/585 nm readout.
- Compare treatment or phenotype groups using matched tissue and cell lysates handling.
- Monitor time-course or pre/post changes in tissue and cell lysates across study conditions.
Interpretation is usually strongest when signal changes are assessed alongside matrix-matched controls, replicate agreement, and the assay's stated analytical window.
Notes for experimental interpretation
- Matrix composition, background signal, and sample handling can influence apparent response; compare like-with-like whenever possible.
- Use appropriate blanks, controls, and replicate wells to distinguish biological differences from plate, reagent, or handling variability.
How should I store my samples?
Fatty acyl-CoAs are not stable and samples should ideally be tested the same day. Otherwise, snap freeze the samples in liquid nitrogen or freeze at -80°C for up to one week.
Can this kit be used with serum or plasma samples?
In our testing, the concentration of fatty acyl-CoAs was too low to detect in these samples.
Can I use a different lysis buffer?
We suggest using a lysis buffer that is pH 7.4 and is 0.5% – 5.0% triton X-100 buffer to dissolve fatty acyl-CoAs.
For laboratories requiring additional technical capacity, we provide scientific support services including assay execution, method guidance, product sourcing, and customization to align the assay with specific experimental objectives. If you need assistance selecting the appropriate kit configuration, adapting the workflow to your application, or identifying related research services, please click Talk to a Scientist, email support@biohippo.com, or review our Research Services; a member of our scientific team will follow up with recommendations tailored to your study.
