EP-CAM Monoclonal Antibody

SKU:BHA12102838
Overview
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EPCAM-specific monoclonal antibody raised in Mouse. Validated for Western Blot, Immunohistochemistry, Immunofluorescence in Human samples. Affinity-purified rabbit IgG.
Specificity Total Protein
Immunogen Synthetic Peptide
UniProt P16422
Gene ID Human: 4072
Applications 4 validated
Options selector
Catalog no. Size
BT-MCA0477-50UL 50 uL
BT-MCA0477-100UL 100 uL
Available Options
Size
Field Specification
Mfr No BT-MCA0477
Alternative Names EPCAM; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial gly
Clonality
  • Monoclonal
Concentration 1 mg/ml
Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Gene ID Human: 4072
Host Mouse
Immunogen Purified recombinant fragment of human EP-CAM expressed in E. Coli.
Isotype
  • IgG
Product Type
  • Primary Antibody
Reactivity
  • Human
Storage -20°C for 1 year
Target EPCAM

Product Overview

Primary Antibody

This antibody targets EPCAM and detects endogenous levels of EPCAM protein.

Validated Applications

Application Dilution Guidance
Western Blot (WB) Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC) Refer to datasheet for recommended working dilution
Immunofluorescence (IF) Refer to datasheet for recommended working dilution
ELISA Refer to datasheet for recommended working dilution
✔ Validated by Bioassay Technology Laboratory

Antibody Specifications

Target EPCAM
Host Mouse
Clonality Monoclonal
Isotype IgG
Reactivity Human
Purification Affinity Purification
Concentration 1 mg/ml
Immunogen Purified recombinant fragment of human EP-CAM expressed in E. Coli.
Formulation Ascitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Storage -20°C for 1 year
Gene ID Human: 4072
UniProt P16422 (Human: P16422; Mouse: Q99JW5)

Scientific Background

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Alternative Names

EPCAM; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial gly

Safety & Handling

⚠ Research Use Only (RUO). Not approved for diagnostic or therapeutic use. Handle with standard laboratory safety precautions. Contains sodium azide — avoid contact with skin/eyes; dispose per local regulations.
TargetEPCAM
HostMouse
ClonalityMonoclonal
IsotypeIgG
ReactivityHuman
PurificationAffinity Purification
Concentration1 mg/ml
ImmunogenPurified recombinant fragment of human EP-CAM expressed in E. Coli.
FormulationAscitic fluid containing 0.03% sodium azide,0.5% BSA, 50%glycerol.
Storage-20°C for 1 year
Gene IDHuman: 4072
UniProtP16422 (Human: P16422; Mouse: Q99JW5)

Optimized working dilutions depend on sample type and detection system. Titration is recommended.

ApplicationDilution Note
Western Blot (WB)Refer to datasheet for recommended working dilution
Immunohistochemistry (IHC)Refer to datasheet for recommended working dilution
Immunofluorescence (IF)Refer to datasheet for recommended working dilution
ELISARefer to datasheet for recommended working dilution

This gene encodes a carcinoma-associated antigen and is a member of a family that includes at least two type I membrane proteins. This antigen is expressed on most normal epithelial cells and gastrointestinal carcinomas and functions as a homotypic calcium-independent cell adhesion molecule. The antigen is being used as a target for immunotherapy treatment of human carcinomas. Mutations in this gene result in congenital tufting enteropathy.

Alternative Names

EPCAM; GA733-2; M1S2; M4S1; MIC18; TACSTD1; TROP1; Epithelial cell adhesion molecule; Ep-CAM; Adenocarcinoma-associated antigen; Cell surface glycoprotein Trop-1; Epithelial cell surface antigen; Epithelial glycoprotein; EGP; Epithelial gly

What species does this antibody react with?
This antibody has been validated for reactivity against Human. Actual cross-reactivity is determined by immunogen sequence homology across species. Contact technical support for unlisted species.
What applications has this antibody been validated for?
Validated applications include: Western Blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA. Each application was tested with positive and negative controls. Contact our team regarding applications not listed.
How was this antibody produced and purified?
This monoclonal IgG antibody was raised in rabbit against a synthesized target-specific peptide. It was affinity-purified from antiserum using an epitope-specific immunogen, yielding a highly specific antibody suitable for multiple research applications.
What is the expected molecular weight of EPCAM?
The expected molecular weight of EPCAM can be found in the product datasheet. Band size may vary slightly depending on post-translational modifications and gel percentage.
What storage conditions does this antibody require?
Store at −20°C for long-term storage. Avoid repeated freeze-thaw cycles. Working aliquots can be stored at 4°C for short-term use. The formulation includes 50% glycerol as a cryoprotectant.

Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.

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Experience the power of Celltrypse™, c-LEcta's innovative enzyme solution for gentle and efficient cell dissociation. Request your free sample and discover a superior alternative for your cell culture workflows.

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