| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human EPS8 recombinant protein (Position: Q255-E802) was used as the immunogen for the EPS8 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EPS8 Antibody / Epidermal growth factor receptor pathway substrate 8 is a anti-EPS8 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunocytochemistry (ICC), Immunofluorescence (IF), Immunoprecipitation (IP), Flow cytometry (FACS), ELISA with listed reactivity in Human. Reported localization: Cytoplasm, plasma membrane.
Key elements and design rationale
- Target: EPS8
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ICC/IF, IP, FACS, ELISA
Biological background
Structurally, EPS8 contains an N-terminal phosphotyrosine-binding (PTB) domain, a central SH3 domain, and a C-terminal effector region responsible for actin bundling and regulation. It belongs to the EPS8 protein family, which includes EPS8L1-3, sharing conserved SH3 domains involved in receptor-proximal signaling. EPS8 interacts with SOS1, ABI1, and E3B1 (ABI2) to form a complex that links receptor activation to Rac-mediated actin remodeling. Known binding partners also include EGFR, SHC, and Grb2, which recruit EPS8 to the plasma membrane upon receptor stimulation.
Functionally, EPS8 regulates actin cytoskeleton organization, cell migration, and proliferation. It enhances Rac GTPase activation and promotes formation of membrane ruffles and lamellipodia during cell movement. EPS8 also acts as a scaffold that coordinates signaling between growth factor receptors and cytoskeletal regulators. In neurons, it supports dendritic spine development and synaptic plasticity. In epithelial tissues, it contributes to cell-cell adhesion and polarity maintenance. Known ligands and substrates include actin filaments and receptor tyrosine kinases such as EGFR and FGFR.
EPS8 expression is dynamically regulated during development and in response to growth factor stimulation. It is highly expressed in embryonic tissues undergoing morphogenesis and wound repair. Dysregulation of EPS8 has been implicated in cancer, where overexpression enhances migration, invasion, and resistance to apoptosis. Elevated EPS8 correlates with aggressive behavior in colorectal, breast, and pancreatic cancers. Pathway involvement includes EGFR signaling, actin cytoskeleton organization, and Ras/Rac-mediated signaling networks.
The EPS8 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
