| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human XPG/ERCC5 recombinant protein (Position: K115-R964) was used as the immunogen for the ERCC5 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ERCC5 Antibody / Excision repair cross-complementation group 5 is a anti-ERCC5 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), Immunocytochemistry (ICC), Flow cytometry (FACS), ELISA with listed reactivity in Human, Mouse, Rat. Reported localization: Nuclear.
Key elements and design rationale
- Target: ERCC5
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ICC/IF, FACS, ELISA
Biological background
ERCC5 is encoded by the ERCC5 gene located on human chromosome 13q33.1. The protein is approximately 118 kilodaltons in size and belongs to the XPG/RAD2 family of structure-specific nucleases. It contains conserved N- and I-domains forming the catalytic core, as well as C-terminal regions mediating interactions with transcription and repair factors such as TFIIH and PCNA. Through these interactions, ERCC5 coordinates incision with repair synthesis during the NER process.
The ERCC5 antibody typically detects a strong nuclear band near 133 kilodaltons by western blot. Immunofluorescence reveals nuclear localization, often enriched at DNA damage foci following UV or oxidative stress exposure. Functional studies demonstrate that ERCC5 excises damaged DNA downstream of the lesion, allowing DNA polymerases to resynthesize the correct strand. Mutations in ERCC5 cause Xeroderma pigmentosum complementation group G (XP-G) and Cockayne syndrome, conditions characterized by UV hypersensitivity, neurological abnormalities, and premature aging.
Beyond its canonical repair function, ERCC5 also contributes to transcription-coupled repair and RNA polymerase II-mediated gene expression. It participates in DNA replication stress responses and protects replication forks from collapse under genotoxic stress. Dysregulation or reduced expression of ERCC5 has been linked to increased cancer susceptibility, particularly skin, liver, and lung cancers.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
