| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived human ESPN recombinant protein (Position: H143-Y854) was used as the immunogen for the ESPN antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ESPN Antibody / Espin is a anti-ESPN Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: ESPN
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, ELISA
Biological background
ESPN is encoded by the ESPN gene located on human chromosome 1p36.31. The protein exists in multiple isoforms ranging from 1,000 to 1,100 amino acids, each containing actin-binding and proline-rich domains that mediate crosslinking of actin filaments. ESPN localizes to microvillar and stereociliary actin cores, where it provides structural reinforcement and length regulation.
The ESPN antibody detects bands between 90 and 110 kilodaltons depending on isoform and shows intense labeling of stereocilia and microvilli under immunofluorescence microscopy. ESPN promotes actin bundle stability by counteracting depolymerizing factors such as cofilin, ensuring the long-term maintenance of hair cell stereocilia. Its overexpression induces elongation of actin protrusions, while loss of function causes disorganization and degeneration of stereocilia, leading to deafness.
Mutations in ESPN are responsible for autosomal recessive deafness and vestibular dysfunction in both humans and animal models. Beyond auditory tissue, ESPN contributes to brush border maintenance in epithelial cells and to filopodia dynamics in neurons and immune cells, reflecting its broad cytoskeletal role.
Because of its crucial function in actin filament assembly and mechanosensory integrity, ESPN serves as a vital biomarker for studies of hearing, balance, and cytoskeletal structure.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
