| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse ETV2 recombinant protein (Position: M1-Q335) was used as the immunogen for the ETV2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
ETV2 Antibody / ETS variant transcription factor 2 is a anti-ETV2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Flow cytometry (FACS), ELISA with listed reactivity in Mouse, Rat.
Key elements and design rationale
- Target: ETV2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, FACS, ELISA
Biological background
ETV2 directly binds to ETS consensus sequences in the promoters of endothelial genes, activating transcription of critical targets such as VEGFR2 (KDR), TIE2, and CDH5 (VE-cadherin). This activity positions ETV2 at the top of the transcriptional hierarchy controlling vasculogenesis and angiogenesis. It cooperates with transcription factors such as GATA2 and FOXC2 to orchestrate endothelial differentiation and vascular remodeling. ETV2 also transiently regulates hematopoietic gene expression, linking early vascular and blood development.
Structurally, ETV2 contains an ETS DNA-binding domain responsible for recognizing GGAA/T motifs in target promoters and a transactivation domain that recruits coactivators such as CBP/p300. These interactions enable the chromatin remodeling necessary for endothelial gene induction. ETV2 belongs to the ETS family of transcription factors, which share highly conserved DNA-binding domains that control diverse developmental and cellular processes.
Functionally, ETV2 plays a crucial role in embryonic vasculogenesis, angiogenesis, and reprogramming of somatic cells toward endothelial fates. Forced expression of ETV2 in fibroblasts can induce endothelial gene expression, demonstrating its potency as a lineage reprogramming factor. ETV2 also participates in VEGF and MAPK signaling pathways, coordinating growth factor responses during vascular morphogenesis. Co-localization studies have shown ETV2 associating with GATA2 and TAL1 in the nucleus, forming transcriptional complexes essential for endothelial specification.
Dysregulation of ETV2 expression disrupts vascular and hematopoietic development and has been implicated in congenital heart defects and embryonic lethality in knockout models. In adult tissues, ETV2 expression is normally repressed, but its reactivation can promote angiogenesis in ischemic and regenerative contexts. Pathway involvement includes VEGF signaling, Notch signaling, and angiogenic transcriptional networks that coordinate vessel growth and stabilization.
Immunohistochemical staining using ETV2 antibody reveals strong nuclear localization in vascular endothelial cells and embryonic tissues. ETV2 antibody from
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Flow cytometry: quantify target-positive populations and signal shifts at single-cell resolution.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
