| Field | Specification |
|---|---|
| Mfr No | |
| Clonality | |
| Host | |
| Immunogen | E.coli-derived mouse EVC2 recombinant protein (Position: Q379-D901) was used as the immunogen for the EVC2 antibody. |
| Isotype | |
| Product Type | |
| Purity | |
| Reactivity | |
| Storage | |
| Target | |
| UniProt # |
Overview
EVC2 Antibody / Ellis-van Creveld syndrome protein 2 is a anti-EVC2 Rabbit antibody Polyclonal (rabbit origin) supplied in Lyophilized format. Recommended for workflows such as Western blot (WB), Immunohistochemistry (IHC), Immunofluorescence (IF), ELISA with listed reactivity in Mouse, Rat. Reported localization: Plasma membrane, Nucleus, Cytoplasm.
Key elements and design rationale
- Target: EVC2
- Antibody details: Rabbit, Polyclonal (rabbit origin), isotype Rabbit IgG
- Format: Lyophilized
- Applications (as listed): WB, IHC, IF, ELISA
Biological background
Functionally, EVC2 antibody identifies a 1,285-amino-acid transmembrane protein localized to the ciliary pocket region. EVC2 forms a complex with EVC, tethering Hedgehog pathway components such as Smoothened and Gli transcription factors at the cilium base to modulate downstream transcriptional activity. It is essential for skeletal morphogenesis and growth plate function.
The EVC2 gene is located on chromosome 4p16.2 and is expressed in ciliated tissues, including cartilage, bone, and embryonic organs. Proper EVC2 function maintains ciliary integrity and ensures controlled Hedgehog pathway activation during tissue differentiation.
Pathologically, mutations in EVC2 cause Ellis-van Creveld syndrome and Weyers acrofacial dysostosis, characterized by short stature, polydactyly, and craniofacial abnormalities. Disruption of EVC2 impairs Hedgehog signaling, leading to developmental malformations. Research using EVC2 antibody supports studies in ciliopathies, developmental biology, and skeletal genetics.
EVC2 antibody is validated for western blotting, immunofluorescence, and immunohistochemistry to detect ciliary and developmental proteins.
Structurally, Ellis-van Creveld syndrome protein 2 is a membrane-tethered scaffold with coiled-coil domains that mediate interaction with EVC and ciliary membrane components. This antibody enables examination of EVC2's function in Hedgehog signaling and developmental regulation.
Research relevance and current trends
- Connecting protein-level changes to phenotype using orthogonal readouts (genetic perturbation, transcriptomics, imaging).
- Considering isoforms and post-translational regulation when interpreting protein-level changes.
- Comparing results across species and model systems with matched controls.
Common research applications
- Western blotting: compare relative abundance and activation-state changes across conditions.
- Immunofluorescence: visualize subcellular distribution and cell-to-cell heterogeneity.
- Immunohistochemistry: map target signal in tissue context and compare regions/phenotypes.
- ELISA: support antibody-based quantification in assay formats where applicable.
Interpret changes in signal alongside appropriate controls and, when relevant, in parallel with total-protein or pathway readouts.
Notes for experimental interpretation
- Signal can reflect expression level, isoform composition, and post-translational state; interpret results in the context of your model system and stimuli.
- Species differences and sample matrices can influence epitope recognition; prioritize matched controls and orthogonal confirmation when feasible.
Antibody notes: Polyclonal antibodies recognize multiple epitopes, which can broaden the epitope footprint and may increase sensitivity in some contexts.
Customization & Add-ons: Can’t find the antibody you need—or require a custom format for your assay? We can help you source the best match or support custom antibody solutions for diverse research needs, including species and isotype selection, conjugations and labeling (e.g., HRP/AP, biotin, fluorophores), purification grade options (Protein A/G, affinity purified), formulation preferences (buffer selection, carrier-free, glycerol-free), custom concentrations and aliquoting, low-endotoxin options for cell-based work, and application-focused QC/validation support (project dependent). Click Talk to a Scientist to submit a request, email us at support@biohippo.com, or explore our Research Services for additional support—our team will follow up with feasibility details and next steps.
